三肽氨肽酶的亚细胞分布检查及其在人血清中的临床应用评估。

Examination of the subcellular distribution of tripeptide aminopeptidase and evaluation of its clinical usefulness in human serum.

作者信息

Kanda S, Maekawa M, Kohno H, Sudo K, Hishiki S, Nakamura S, Kanno T

出版信息

Clin Biochem. 1984 Aug;17(4):253-7. doi: 10.1016/s0009-9120(84)90167-x.

Abstract

Following electrophoretic separation, we assayed tripeptide aminopeptidase (EC 3.4.11.4) in human tissues and sera free of interferences by other aminopeptidases. Tripeptide aminopeptidase is distributed in various human tissues, with the highest activity observed in liver and lymphocytes. The highest specific activity of the enzyme was observed in the soluble fraction prepared from liver, and 65% of the enzyme activity in the original homogenate was recovered in this fraction. Cancerous and fetal tissues showed lower enzyme activities than normal adult tissues. Elevations of tripeptide aminopeptidase were observed in sera of patients with liver disorders, leukemias, and autoimmune diseases.

摘要

电泳分离后，我们在不受其他氨肽酶干扰的人体组织和血清中测定了三肽氨肽酶（EC 3.4.11.4）。三肽氨肽酶分布于人体的各种组织中，在肝脏和淋巴细胞中活性最高。该酶的最高比活性在肝脏制备的可溶性部分中观察到，原始匀浆中65%的酶活性在该部分中得以回收。癌组织和胎儿组织的酶活性低于正常成人组织。在患有肝脏疾病、白血病和自身免疫性疾病的患者血清中观察到三肽氨肽酶升高。