García-Marín J J, Esteller A
Clin Sci (Lond). 1984 Nov;67(5):499-504. doi: 10.1042/cs0670499.
The interference between biliary phospholipid and bilirubin secretion was investigated in rats with bile fistulae, under conditions of normal and maximal bilirubin secretion. The enterohepatic circulation of bile salts was interrupted and the animals received infusions of sodium taurocholate, a micelle-forming physiological bile salt. Sodium taurocholate infusion (0.19 mumol min-1 100 g-1 body weight) induced an increase in bile flow and phospholipid secretion, while basal bilirubin secretion was not increased. Bilirubin infusion (0.26 mumol min-1 100 g-1 body weight) induced a decrease in basal and taurocholate-stimulated phospholipid secretion. Biliary mixed micelle formation was presumably altered during bilirubin infusion, although bile taurocholate concentration, taurocholate secretion rate and bile flow were not modified. When sodium taurocholate was infused during bilirubin-decreased phospholipid secretion, this secretion was restored but maximal biliary bilirubin secretion was not increased. These results provide circumstantial evidence for the hypothesis that mixed micelle formation is not an important determinant of maximal bilirubin transport into bile.
在具有胆瘘的大鼠中，于正常和最大胆红素分泌条件下，研究了胆汁磷脂与胆红素分泌之间的干扰。胆汁盐的肠肝循环被中断，并且给动物输注牛磺胆酸钠，一种形成微胶粒的生理性胆汁盐。输注牛磺胆酸钠（0.19 μmol·min⁻¹·100 g⁻¹体重）导致胆汁流量和磷脂分泌增加，而基础胆红素分泌未增加。输注胆红素（0.26 μmol·min⁻¹·100 g⁻¹体重）导致基础和牛磺胆酸盐刺激的磷脂分泌减少。尽管胆汁牛磺胆酸盐浓度、牛磺胆酸盐分泌速率和胆汁流量未改变，但在输注胆红素期间，胆汁混合微胶粒的形成可能发生了改变。当在胆红素降低磷脂分泌期间输注牛磺胆酸钠时，这种分泌得以恢复，但最大胆汁胆红素分泌未增加。这些结果为混合微胶粒形成不是胆红素最大转运入胆汁的重要决定因素这一假说提供了间接证据。
