Ottesen B, Hansen B, Fahrenkrug J, Fuchs A R
Endocrinology. 1984 Oct;115(4):1648-50. doi: 10.1210/endo-115-4-1648.
The concentration of vasoactive intestinal peptide (VIP) was measured by RIA in pituitary extracts of female cats; it was significantly higher in the posterior than in the anterior lobe, 47.3 +/- 3.9 pmol/g wet wt vs. 7.7 +/- 2.0 pmol/g wet wt (mean +/- SE, n = 5 in both instances). To investigate the effect of VIP on the release of posterior pituitary hormones, the concentration of arginine vasopressin and oxytocin was measured by RIA in jugular vein plasma during intracarotid infusion of VIP. The levels of both hormones rose during the 5-min infusion of VIP. Oxytocin levels increased from a mean of 7.9 microU/ml to a maximum of 34.9 microU/ml at 1 min and arginine vasopressin levels from from 27.4 microU/ml to a maximum of 157 microU/ml at 1 min. The levels of both hormones returned to baseline values in about 20 min. Since VIP has been localized to the nerve terminals of the neurohypophysis, these data suggest that endogenous VIP may play a role as a neurotransmitter in the magnocellular hypothalmo-neurosecretory system.
采用放射免疫分析法（RIA）测定了雌性猫垂体提取物中血管活性肠肽（VIP）的浓度；结果显示，垂体后叶中的VIP浓度显著高于前叶，分别为47.3±3.9 pmol/g湿重和7.7±2.0 pmol/g湿重（均值±标准误，两种情况均为n = 5）。为研究VIP对垂体后叶激素释放的影响，在颈内动脉注入VIP期间，采用RIA测定颈静脉血浆中精氨酸加压素和催产素的浓度。在注入VIP的5分钟内，两种激素的水平均升高。催产素水平从平均7.9微单位/毫升在1分钟时升至最高34.9微单位/毫升，精氨酸加压素水平从27.4微单位/毫升在1分钟时升至最高157微单位/毫升。两种激素的水平在约20分钟后恢复至基线值。由于VIP已被定位到神经垂体的神经末梢，这些数据表明内源性VIP可能在大细胞下丘脑 - 神经分泌系统中作为神经递质发挥作用。
