Meehan R R, Barlow D P, Hill R E, Hogan B L, Hastie N D
EMBO J. 1984 Aug;3(8):1881-5. doi: 10.1002/j.1460-2075.1984.tb02062.x.
We have shown by molecular hybridisation that the mRNAs for albumin, transferrin, apolipoprotein-A1, and alpha 1-antitrypsin are expressed at high levels in mouse visceral yolk sac. In contrast, the mRNAs for contrapsin (a plasma protease inhibitor) and the major urinary proteins (MUPs) are not detected in the visceral yolk sac at any stage of embryonic development. Contrapsin and MUP mRNAs both appear late in liver development. These differences in expression suggest that the visceral yolk sac is more similar to the foetal than adult mouse liver in its pattern of gene expression. However, the developmental time course of expression of these mRNAs is different between the foetal liver and the yolk sac. Evidence is also presented that the visceral yolk sac synthesises and secretes other apolipoproteins in addition to apolipoprotein-A1. These results suggest that the visceral yolk sac and foetal liver, two tissues with different embryological lineages, perform similar functions but are independently programmed for expression of the same set of serum protein genes.
我们通过分子杂交表明，白蛋白、转铁蛋白、载脂蛋白A1和α1-抗胰蛋白酶的mRNA在小鼠内脏卵黄囊中高水平表达。相比之下，在胚胎发育的任何阶段，内脏卵黄囊中均未检测到抗胰蛋白酶（一种血浆蛋白酶抑制剂）和主要尿蛋白（MUPs）的mRNA。抗胰蛋白酶和MUP的mRNA均在肝脏发育后期出现。这些表达差异表明，内脏卵黄囊在基因表达模式上比成年小鼠肝脏更类似于胎儿肝脏。然而，这些mRNA在胎儿肝脏和卵黄囊之间的表达发育时间进程是不同的。也有证据表明，内脏卵黄囊除了合成和分泌载脂蛋白A1外，还合成和分泌其他载脂蛋白。这些结果表明，内脏卵黄囊和胎儿肝脏这两个具有不同胚胎谱系的组织，执行相似的功能，但在表达同一组血清蛋白基因方面是独立编程的。
