Barth R K, Gross K W, Gremke L C, Hastie N D
Proc Natl Acad Sci U S A. 1982 Jan;79(2):500-4. doi: 10.1073/pnas.79.2.500.
The mouse liver contains a group of 10--12 different tissue-specific mRNAs, each present at an average concentration of 12,000--15,000 copies per cell [Hastie, N. D. & Bishop, J. O. (1976) Cell 9, 761--774]. We have determined, by translation in vitro, that these mRNAs are developmentally regulated in the liver. We have also used specific cloned probes to quantitate the developmental time course of expression of five different abundant liver mRNAs. We have found that there are at least three periods during liver development when specific abundant mRNAs are first detectable: prior to 14 days postconception, at birth, and during the onset of sexual maturity. These results indicate that all the members of this mRNA group are not under common developmental regulation. One of the abundant liver mRNAs (p54 mRNA) increases more than 1000-fold in the liver 1 day before birth. We discuss factors that may be involved in the developmental regulation of expression of the genes encoding these mRNAs.
小鼠肝脏含有一组10 - 12种不同的组织特异性mRNA，每个mRNA在每个细胞中的平均浓度为12000 - 15000拷贝[哈斯蒂，N.D. & 毕晓普，J.O.（1976年）《细胞》9卷，761 - 774页]。我们通过体外翻译确定，这些mRNA在肝脏中受到发育调控。我们还使用了特定的克隆探针来定量五种不同的丰富肝脏mRNA的表达发育时间进程。我们发现，在肝脏发育过程中至少有三个时期，特定的丰富mRNA首次可检测到：受孕后14天之前、出生时以及性成熟开始时。这些结果表明，该mRNA组的所有成员并非都受到共同的发育调控。其中一种丰富的肝脏mRNA（p54 mRNA）在出生前1天在肝脏中的含量增加了1000多倍。我们讨论了可能参与这些mRNA编码基因表达发育调控的因素。
