鸟氨酸脱羧酶的无细胞合成。硫代乙酰胺处理大鼠肝脏中mRNA活性的变化。

Cell-free synthesis of ornithine decarboxylase. Changes in mRNA activity in the liver of thioacetamide-treated rats.

作者信息

Kameji T, Fujita K, Noguchi T, Takiguchi M, Mori M, Tatibana M, Hayashi S

出版信息

Eur J Biochem. 1984 Oct 1;144(1):35-9. doi: 10.1111/j.1432-1033.1984.tb08427.x.

DOI:10.1111/j.1432-1033.1984.tb08427.x

Abstract

Ornithine decarboxylase (ODC)mRNA associated with free polysomes of rat liver was translated in a reticulocyte lysate cell-free system. Newly synthesized ODC protein was identified by specific immunoprecipitation, molecular size as determined by polyacrylamide gel electrophoresis with sodium dodecyl sulfate, and competition by excess unlabeled ODC in the immunoprecipitation. A single injection of thioacetamide was found to cause several fold increases in both immunotitratable ODC protein and polysomal ODC-mRNA activity, while it provoked a much larger increase in ODC activity in rat liver. The results indicate that the induction of hepatic ODC activity by thioacetamide treatment is due not only to an increase in the activity of polysomal ODC-mRNA but also to a translational and/or posttranslational control.

摘要

与大鼠肝脏游离多核糖体相关的鸟氨酸脱羧酶（ODC）mRNA在网织红细胞裂解物无细胞系统中进行翻译。通过特异性免疫沉淀鉴定新合成的ODC蛋白，用十二烷基硫酸钠聚丙烯酰胺凝胶电泳测定分子大小，并在免疫沉淀中用过量未标记的ODC进行竞争。发现单次注射硫代乙酰胺会使免疫可滴定的ODC蛋白和多核糖体ODC-mRNA活性均增加数倍，同时它在大鼠肝脏中引起ODC活性更大幅度的增加。结果表明，硫代乙酰胺处理诱导肝脏ODC活性不仅是由于多核糖体ODC-mRNA活性的增加，还归因于翻译和/或翻译后控制。