Neumann D, Scharf K D, Nover L
Eur J Cell Biol. 1984 Jul;34(2):254-64.
Treating tomato cell cultures and leaves by a physiological heat shock (hs) at 35 to 39 degrees C results in a progressive disintegration of the nucleolus and the assembly of cytoplasmic hs granules. Other ultrastructural changes are not observed. The alterations of the nucleoli coincide with an immediate stop of the processing and with a strongly decreased synthesis of pre-rRNA. Both hs effects are reversed after shift-down to normal temperature conditions (25 degrees C). Assembly of cytoplasmic hs granules depends on the accumulation of the newly forming hs proteins and on supraoptimal temperatures. It is not observed in preinduced cultures synthesizing hs proteins at 25 degrees C. Autoradiographic studies reveal the preferential accumulation of hsp in the nucleoli and hs granules. Furthermore uridine labeling points to the presence of RNA in electron dense particles of both subcellular components. A survey on the state of hsp synthesis and structural binding as well as on the ultrastructural changes is given for 12 selected hs regimes.
在35至39摄氏度对番茄细胞培养物和叶片进行生理性热激（hs）处理，会导致核仁逐渐解体并形成细胞质热激颗粒。未观察到其他超微结构变化。核仁的改变与加工过程立即停止以及前体核糖体RNA合成大幅减少同时发生。在转移至正常温度条件（25摄氏度）后，这两种热激效应均可逆转。细胞质热激颗粒的形成取决于新形成的热激蛋白的积累以及超适宜温度。在25摄氏度合成热激蛋白的预诱导培养物中未观察到这种情况。放射自显影研究显示热激蛋白优先积累在核仁和热激颗粒中。此外，尿苷标记表明这两种亚细胞成分的电子致密颗粒中存在RNA。针对12种选定的热激处理方案，给出了热激蛋白合成和结构结合状态以及超微结构变化的综述。
