Weber C, Pernis B, Ting W, Rosenkrantz K, Reemtsma K
Diabetologia. 1984 Jul;27 Suppl:160-2. doi: 10.1007/BF00275678.
Major histocompatibility complex-linked immune response genes are thought to influence susceptibility to induction of both human insulin-dependent diabetes and murine streptozotocin-induced diabetes. To clarify this relationship, we administered streptozotocin intravenously in two doses (120 and 240 mg/kg body weight) on days 0 and 14, and monitored blood glucose until day 100 in young adult male mice of differing background genome and/or H-2 complex. In addition, we examined the effect of allogeneic whole blood transfusion on subsequent susceptibility to diabetes. B10 recombinant mice possessing the k allele at the centromeric H-2-K and I-A loci were most susceptible to diabetes induction. Variation in susceptibility of different inbred strains with the same major histocompatibility complex genotype suggested a rôle for non-major histocompatibility complex genes. Blood transfusion delayed the onset, but did not significantly reduce the incidence of, delayed hyperglycaemia. We conclude that, in this murine model, multiple genes within the outside the major histocompatibility complex influence multiple-dose streptozotocin-diabetes susceptibility, and that prior blood transfusion may modulate diabetes induction.
主要组织相容性复合体相关的免疫反应基因被认为会影响人类胰岛素依赖型糖尿病和小鼠链脲佐菌素诱导糖尿病的易感性。为了阐明这种关系，我们在第0天和第14天给不同背景基因组和/或H-2复合体的年轻成年雄性小鼠静脉注射两剂链脲佐菌素（120和240毫克/千克体重），并监测血糖直至第100天。此外，我们研究了同种异体全血输血对后续糖尿病易感性的影响。在着丝粒H-2-K和I-A位点具有k等位基因的B10重组小鼠对糖尿病诱导最敏感。具有相同主要组织相容性复合体基因型的不同近交系易感性的差异表明非主要组织相容性复合体基因发挥了作用。输血延迟了迟发性高血糖的发作，但并未显著降低其发生率。我们得出结论，在这个小鼠模型中，主要组织相容性复合体外的多个基因影响多剂量链脲佐菌素诱导糖尿病的易感性，并且预先输血可能调节糖尿病诱导。
