Kanai K, Kanamura S, Asada-Kubota M, Watanabe J, Oka M
Gastroenterology. 1984 Nov;87(5):1131-7.
The smooth endoplasmic reticulum in hepatocytes of early postnatal and adult ddY male mice injected with phenobarbital was analyzed stereologically. The animals received daily intraperitoneal injections of phenobarbital and were killed about 24 h after the last injection. In early postnatal animals (injected with 35 mg/kg of the drug at days 1 and 2 after birth, or injected with 35 mg/kg at day 2 and with 50 mg/kg at days 3 and 4 after birth), the smooth endoplasmic reticulum proliferated both in perivenular and periportal hepatocytes; there was no significant difference in amount of smooth endoplasmic reticulum between the cells of the two zones. In adult animals, however, proliferation occurred only in perivenular cells after the administration of 35 mg/kg of phenobarbital for 2 days, and predominantly in perivenular cells even after the administration of 150 mg/kg of the drug for 7 days. These results suggest that the conspicuous proliferation of the smooth endoplasmic reticulum in perivenular hepatocytes in response to phenobarbital administration, which is characteristic of adult liver, does not occur in early postnatal mice but becomes manifest during postnatal development.
对出生后早期和成年ddY雄性小鼠注射苯巴比妥后肝细胞中的滑面内质网进行了体视学分析。动物每日腹腔注射苯巴比妥，并在最后一次注射后约24小时处死。在出生后早期的动物中（出生后第1天和第2天注射35mg/kg药物，或出生后第2天注射35mg/kg，第3天和第4天注射50mg/kg），汇管区周围和门管区周围的肝细胞中滑面内质网均有增殖；两个区域细胞的滑面内质网数量无显著差异。然而，在成年动物中，给予35mg/kg苯巴比妥2天后，仅在汇管区周围细胞中出现增殖，即使给予150mg/kg药物7天后，也主要在汇管区周围细胞中出现增殖。这些结果表明，成年肝脏中典型的对苯巴比妥给药反应的汇管区周围肝细胞中滑面内质网的显著增殖在出生后早期小鼠中并不发生，而是在出生后发育过程中才表现出来。
