Kanai K, Watanabe J, Fujimoto S, Kanamura S
Department of Anatomy, Kansai Medical University, Osaka, Japan.
Exp Toxicol Pathol. 1993 Aug;45(4):199-203. doi: 10.1016/S0940-2993(11)80387-8.
Smooth endoplasmic reticulum (SER) proliferation after phenobarbital (PB) administration has been described to occur predominantly in perivenular hepatocytes. We analyzed by quantitative electron microscopy changes in ER amounts in periportal, midzonal and perivenular hepatocytes from mice injected with 35, 50, 100 or 150 mg/kg of PB once a day for 3 days. The SER proliferated in midzonal hepatocytes in addition to perivenular hepatocytes even when low doses (35 or 50 mg/kg) were administered, and in hepatocytes of all three zones in case of high doses (100 or 150 mg/kg). Moreover, net proliferation of SER after administration of an amount of PB was not different in hepatocytes of any zones where the proliferation occurred, except animals injected with 100 mg/kg. The results suggest that there is no difference in SER producing capacity in hepatocytes of three zones when the cells have recognized the stimulation of PB.
苯巴比妥（PB）给药后，滑面内质网（SER）的增殖主要发生在肝小叶中央静脉周围的肝细胞中。我们通过定量电子显微镜分析了每天注射35、50、100或150mg/kg PB，连续3天的小鼠肝门周、中区和中央静脉周围肝细胞内质网数量的变化。即使给予低剂量（35或50mg/kg），SER除了在中央静脉周围的肝细胞中增殖外，还在中区肝细胞中增殖；而给予高剂量（100或150mg/kg）时，SER在所有三个区带的肝细胞中均有增殖。此外，除了注射100mg/kg的动物外，在发生增殖的任何区带的肝细胞中，给予一定量PB后SER的净增殖没有差异。结果表明，当细胞识别到PB的刺激时，三个区带的肝细胞在SER产生能力上没有差异。
