沃纳综合征中大分子酸性糖胺聚糖的尿排泄情况

Urinary excretion of macromolecular acidic glycosaminoglycans in Werner's syndrome.

作者信息

Goto M, Murata K

出版信息

Clin Chim Acta. 1978 Apr 17;85(2):101-6. doi: 10.1016/0009-8981(78)90227-9.

Abstract

The urinary acidic glycosaminoglycans (AGAG) of Werner's syndrome were isolated, purified and characterized by gel-chromatography, cellulose acetate electrophoresis, chemical analysis, streptomyces hyaluronidase susceptibility and viscometry. The AGAG appeared at the first peaks of the 0.6 M and 0.8 M fractions obtained through Sephadex G-100 were mainly a a hyaluronic acid (HA). HA was composed of 16% of the urinary AGAG. The AGAG at the first peak had the maximum molecular weight of 360 000 in the 0.8 M fraction followed by lesser molecular weights in the other fractions.

摘要

对沃纳综合征患者的尿酸性糖胺聚糖（AGAG）进行了分离、纯化，并通过凝胶色谱法、醋酸纤维素电泳、化学分析、链霉菌透明质酸酶敏感性测试和粘度测定对其进行了表征。通过葡聚糖凝胶G-100获得的0.6M和0.8M级分的第一个峰处出现的AGAG主要是透明质酸（HA）。HA占尿AGAG的16%。在0.8M级分中第一个峰处的AGAG的最大分子量为360000，其他级分中的分子量较小。