Klump H H, Falk H
Hoppe Seylers Z Physiol Chem. 1984 Jun;365(6):661-5. doi: 10.1515/bchm2.1984.365.1.661.
As was shown in a recent paper [Klump, H. & Hütig, H. (1980) Ber. Bunsenges. Phys. Chem. 84, 250-253] by one of the authors (H.K.), nucleosome-like histone complexes can be formed on ribopolynucleotides as well as on deoxyribopolynucleotides. The conditions used for complex formation have to be such that single strands and triple strands as secondary structures of the polynucleotides employed are excluded under all ionic-strength conditions. There is clear evidence however for ordered poly(U)/histone complexes. In this paper we further characterize these poly(U) complexes and present electron micrographs of both DNA histone complexes and poly(U) histone complexes to show their close resemblance.
正如其中一位作者(H.K.)近期发表的一篇论文[克伦普,H. & 胡蒂希,H.(1980年)《德国化学会会志:物理化学》84卷,第250 - 253页]所示,核小体样组蛋白复合物既能在核糖多核苷酸上形成,也能在脱氧核糖多核苷酸上形成。形成复合物所采用的条件必须确保,在所使用的所有离子强度条件下,作为多核苷酸二级结构的单链和三链均被排除。然而,有明确证据表明存在有序的聚(U)/组蛋白复合物。在本文中,我们进一步对这些聚(U)复合物进行表征,并展示DNA组蛋白复合物和聚(U)组蛋白复合物的电子显微镜照片,以显示它们的高度相似性。