Kalland T
Int J Immunopharmacol. 1984;6(4):299-305. doi: 10.1016/0192-0561(84)90046-8.
The effect of the chemical carcinogen 3-methylcholanthrene (3-MCA) on mouse natural killer (NK) cells was tested in vitro applying kinetic analysis of the NK-tumor cell interaction. 3-MCA inhibited the lytic activity of spleen cells against YAC-1 target cells in a dose-dependent manner. Maximal inhibition, of Vmax was about 60% and a parallell decrease in KM values was observed. Inhibition was observed after 30 min preincubation of effector cells while pre-incubation of target cells had no effect. Inclusion of Indomethacin in the assay to exclude the possibility that prostaglandins induced by 3-MCA were the mediators of NK suppression had no effect. 3-MCA inhibited basal and interferon-activated NK lysis to about the same extent, while Vmax and KM values of effector cells pretreated with 3-MCA and assayed in the presence of interferon was close to control values. In a single cell assay for NK activity it was found that 3-MCA interfered with the lytic step of NK mediated lysis without affecting target binding. These data are in accordance with an earlier observation that a single injection of 3-MCA transiently inhibited mouse NK cells in vivo and evidence is presented that 3-MCA inhibits the lytic activity of a subpopulation of NK cells. The effects of 3-MCA were reversed by interferon.
应用自然杀伤（NK）细胞与肿瘤细胞相互作用的动力学分析方法，在体外检测了化学致癌物3-甲基胆蒽（3-MCA）对小鼠NK细胞的影响。3-MCA以剂量依赖的方式抑制脾细胞对YAC-1靶细胞的裂解活性。最大抑制时，Vmax约为60%，同时观察到KM值平行下降。效应细胞预孵育30分钟后出现抑制，而靶细胞预孵育则无影响。在检测中加入消炎痛以排除3-MCA诱导的前列腺素是NK抑制介质的可能性，但没有效果。3-MCA对基础NK裂解和干扰素激活的NK裂解的抑制程度大致相同，而用3-MCA预处理并在干扰素存在下检测的效应细胞的Vmax和KM值接近对照值。在一项针对NK活性的单细胞检测中发现，3-MCA干扰了NK介导的裂解的裂解步骤，而不影响靶细胞结合。这些数据与早期观察结果一致，即单次注射3-MCA可在体内短暂抑制小鼠NK细胞，并且有证据表明3-MCA抑制了NK细胞亚群的裂解活性。3-MCA的作用可被干扰素逆转。
