Schroyens W, Bruyneel R, Tchao R, Leighton J, Dragonetti C, Mareel M
Invasion Metastasis. 1984;4(3):160-70.
Two epithelial cell lines of urological origin have been compared for their invasiveness in an in vitro three-dimensional culture system, using embryonic chick cardiac muscle as host tissue. Cells from the Nara bladder tumor line (NBT-II), an invasive tumor in the rat, invaded and progressively occupied the cardiac muscle which degenerated. Cells from a dog kidney line (MDCK), which are of low tumorigenicity in nude mice, failed to invade into the cardiac muscle in vitro. MDCK cells formed a structurally polarized epithelium around the heart tissue. MDCK is the first established epithelial cell line that has been found to grow in this invasion assay culture system and yet did not invade the heart fragment.
利用鸡胚心肌作为宿主组织，在体外三维培养系统中比较了两种泌尿学来源的上皮细胞系的侵袭性。来自奈良膀胱肿瘤细胞系（NBT-II）的细胞，该细胞系在大鼠体内是侵袭性肿瘤，能侵入并逐渐占据发生退化的心肌。来自犬肾细胞系（MDCK）的细胞，在裸鼠中致瘤性较低，在体外未能侵入心肌。MDCK细胞在心脏组织周围形成了结构极化的上皮。MDCK是首个在这种侵袭试验培养系统中生长但未侵入心脏组织碎片的已建立上皮细胞系。
