Yu D, Gao J
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1994 Aug;16(4):317-22 inside backcover.
Using limiting dilution assay, variants were cloned from the CNE-2Z cell line. The variants CNE2L2, CNE2H2, CNE2L4 and CNE2M2, with cell electrophoresis rates which differed greatly, were assayed for their invasive capabilities by observing their ability to invade preculture embryonic chicken heart fragment (PHF) in organ culture using a gyrotory shaker system. The results indicated that CNE2L2 variant cells invaded 9-day-old chicken embryonic heart fragments at a rate significantly higher than those seen using CNE2L4 and CNE2M2 in 7-day assay. Computer image analysis showed that the CNE2L2 cells occupied 80.2% of the PHF area much greater than that occupied by CNE2L4 (46.5%) or CNE2M2 (36.5%). This experiment also showed that the electrophoresis rate of the CNE2Z variants was positively related to their invasive capabilities in vitro.
采用有限稀释法从CNE - 2Z细胞系中克隆出变异体。通过观察变异体CNE2L2、CNE2H2、CNE2L4和CNE2M2在旋转振荡器系统的器官培养中侵袭预培养的鸡胚心脏组织块（PHF）的能力，检测其侵袭能力，这些变异体的细胞电泳速率差异很大。结果表明，在7天的实验中，CNE2L2变异体细胞侵袭9日龄鸡胚心脏组织块的速率显著高于CNE2L4和CNE2M2。计算机图像分析显示，CNE2L2细胞占据PHF面积的80.2%，远大于CNE2L4（46.5%）或CNE2M2（36.5%）占据的面积。该实验还表明，CNE2Z变异体的电泳速率与其体外侵袭能力呈正相关。
