McGovern E P, Swynnerton N F, Steele P D, Mangold D J
Int J Radiat Oncol Biol Phys. 1984 Sep;10(9):1517-20. doi: 10.1016/0360-3016(84)90493-0.
A high pressure liquid chromatography (HPLC) plasma assay for WR-1065 is described which is both precise (coefficient of variation less than 5%) and accurate (% average deviation less than or equal to 6.1) throughout the concentration range from 1 to 500 micrograms/mL of plasma. The analyte is separated by HPLC and detected with a thiol specific electrochemical transducer cell. The detector response is linear over the ranges 1 to 10 micrograms/mL (R2 = 0.995), 10 to 100 micrograms/mL (R2 = 0.995), and 100 to 500 micrograms/mL (R2 = 0.974). The absolute retention times for WR-1065 and WR-1729 are 9 and 12 minutes, respectively. The assay uses 100 microL of plasma and requires a total chromatography cycle time of 40 minutes. The method has been found suitable for the determination of WR-1065 in plasma from a beagle dog after i.v. administration of S-2-(3-aminopropylamino)ethyl phosphorothioate (WR-2721).
本文描述了一种用于测定WR-1065的高压液相色谱（HPLC）血浆检测方法，该方法在血浆浓度范围为1至500微克/毫升时，既精确（变异系数小于5%）又准确（平均偏差百分比小于或等于6.1）。分析物通过HPLC分离，并用硫醇特异性电化学传感池进行检测。检测器响应在1至10微克/毫升（R2 = 0.995）、10至100微克/毫升（R2 = 0.995）和100至500微克/毫升（R2 = 0.974）范围内呈线性。WR-1065和WR-1729的绝对保留时间分别为9分钟和12分钟。该检测方法使用100微升血浆，总色谱循环时间为40分钟。已发现该方法适用于在静脉注射S-2-(3-氨丙基氨基)乙基硫代磷酸酯（WR-2721）后，测定比格犬血浆中的WR-1065。
