Fahey R C, Newton G L
Int J Radiat Oncol Biol Phys. 1985 Jun;11(6):1193-7. doi: 10.1016/0360-3016(85)90069-0.
Alkaline phosphatase was used in developing two new assays for WR-2721; one involved simple spectrophotometric titration with Ellman's reagent and the other was based upon HPLC analysis of a monobromobimane derivative. Both methods gave acceptable results when applied to whole plasma. Assays for WR-1065 and disulfide forms of the drug were also developed but were found to give unreliable results with whole plasma owing to apparent rapid reaction of these drug forms with plasma constituents. The methods were applied to the analysis of blood samples in rat 0-70 min after i.v. injection of WR-2721.
碱性磷酸酶被用于开发两种针对WR-2721的新检测方法；一种是用埃尔曼试剂进行简单的分光光度滴定，另一种基于对单溴代双硫腙衍生物的高效液相色谱分析。当应用于全血时，两种方法都给出了可接受的结果。还开发了针对WR-1065及其二硫键形式药物的检测方法，但发现由于这些药物形式与血浆成分的明显快速反应，应用于全血时结果不可靠。这些方法被应用于静脉注射WR-2721后0至70分钟大鼠血样的分析。
