McDowall A W, Hofmann W, Lepault J, Adrian M, Dubochet J
J Mol Biol. 1984 Sep 5;178(1):105-11. doi: 10.1016/0022-2836(84)90233-x.
Frozen hydrated sections of vitrified unstained insect flight muscle in the rigor and relaxed states were studied by cryo-electron microscopy. High resolution images with good contrast were obtained. As judged from direct images and from the comparison of optical diffractograms with X-ray diffraction patterns obtained from glycerinated muscle fibres, the vitrified specimen is well preserved. Preparation artifacts encountered in conventional plastic sections are avoided. The preservation of the relaxed structure also seems better than in freeze-etched replicas as the 14.5 nm repeat of crossbridges on thick filaments is easily visible.
通过冷冻电子显微镜研究了处于僵直和松弛状态的玻璃化未染色昆虫飞行肌的冷冻水合切片。获得了具有良好对比度的高分辨率图像。从直接图像以及光学衍射图与甘油处理的肌纤维的X射线衍射图谱的比较判断，玻璃化标本保存良好。避免了传统塑料切片中遇到的制备假象。由于粗肌丝上14.5nm的横桥重复很容易看到，松弛结构的保存似乎也比冷冻蚀刻复制品更好。
