Velky T S, Yang J C, Greenburg A G
J Trauma. 1984 Sep;24(9):824-9. doi: 10.1097/00005373-198409000-00008.
Plasma fibronectin (PFN) is a high molecular weight adhesive glycoprotein. Following trauma or sepsis PFN is acutely depleted, with rapid restoration of normal or supranormal levels after 24 to 48 hours. Cecal ligation with perforation provides an animal model of surgical trauma combined with polymicrobial sepsis. The present study examines whether this PFN level restoration 6 to 24 hours postoperatively is associated with de novo PFN synthesis and how this response is altered by pre-existing protein-calorie deprivation. Thirty-six adult male rats were divided into four groups: I--Controls, II--Prefasted Controls, III--Ligated, IV--Prefasted and Ligated. Control and experimental groups received intracardiac 35S-methionine 2 hours postoperatively. Plasma fibronectin (PFN) levels, PFN specific activity, plasma total protein, and total protein specific activity were determined at 0, 6, 24, and 48 hours postoperatively. Ligated rats (Groups III & IV) demonstrated significant PFN level increases 24 to 48 hours postoperatively (p less than 0.01-0.05). Despite a significant preoperative PFN level depression in prefasted rats (Groups II & IV), the 24-48 hours response to cecal ligation was not significantly altered. PFN specific activity was significantly increased among the operative groups 6 hours postoperatively, demonstrating de novo PFN synthesis following cecal ligation and perforation.
血浆纤维连接蛋白（PFN）是一种高分子量黏附糖蛋白。创伤或脓毒症后，PFN会急剧减少，24至48小时后会迅速恢复至正常或超正常水平。盲肠结扎穿孔术可提供一种手术创伤合并多微生物脓毒症的动物模型。本研究探讨术后6至24小时这种PFN水平的恢复是否与PFN的重新合成有关，以及这种反应如何因先前存在的蛋白质 - 热量缺乏而改变。36只成年雄性大鼠被分为四组：I组——对照组，II组——术前禁食对照组，III组——结扎组，IV组——术前禁食并结扎组。对照组和实验组在术后2小时接受心内注射35S - 蛋氨酸。在术后0、6、24和48小时测定血浆纤维连接蛋白（PFN）水平、PFN比活性、血浆总蛋白和总蛋白比活性。结扎大鼠（III组和IV组）在术后24至48小时显示出PFN水平显著升高（p < 0.01 - 0.05）。尽管术前禁食大鼠（II组和IV组）的PFN水平显著降低，但对盲肠结扎的24 - 48小时反应未发生显著改变。术后6小时，手术组的PFN比活性显著增加，表明盲肠结扎穿孔术后有PFN的重新合成。
