Saito I, Hisanaga N, Takeuchi Y, Ono Y, Iwata M, Masuda K, Gotoh M, Matsumoto T, Fukaya Y, Okutani H
Sangyo Igaku. 1984 Jan;26(1):15-21.
Pest control operators usually spray pesticides in small areas such as a kitchen in a restaurant and are exposed to various pesticides, especially those of the organophosphorus (OP) type. In order to evaluate their occupational exposure to OP pesticides during the work, OP pesticides in blood and alkyl phosphate metabolites in urine of these operators were analyzed and the relationship between pesticide exposure and analytical results were studied. OP pesticides in blood were analyzed by gas chromatography with flame photometric detector (FPD-GC) after separation of phospholipid in blood with silicagel column chromatography. OP pesticides were not detected in any blood samples (the limit of detection was 1 ng/ml). Dimethylphosphate (DMP) and dimethylthiophosphate (DMTP), being urinary metabolites of OP pesticides, were analyzed by FPD-GC after benzyl derivatization. This method eliminated interfering peaks in gas chromatograms. The ratio of two isomeric derivatives of DMTP was found to be constant. Both DMP and DMTP of the exposed group were significantly higher than those of the non-exposed group, DMP being higher than DMTP. The ratio of DMP to DMTP in the fenitrothion-dichlorvos-exposed group was significantly higher than that in the fenitrothion-exposed group. It was considered that the ratio might reflect a result of pesticide exposure. The urinary metabolites of OP pesticides tended to become lower with the lapse of time since the last exposure. However, small amounts were detected in a few samples even 5 days after the last exposure.
害虫防治操作人员通常在诸如餐厅厨房等小区域喷洒农药，会接触到各种农药，尤其是有机磷（OP）类农药。为了评估他们在工作期间对OP农药的职业暴露情况，对这些操作人员血液中的OP农药和尿液中的烷基磷酸酯代谢物进行了分析，并研究了农药暴露与分析结果之间的关系。血液中的OP农药通过硅胶柱色谱法分离血液中的磷脂后，采用带火焰光度检测器的气相色谱法（FPD-GC）进行分析。在任何血液样本中均未检测到OP农药（检测限为1 ng/ml）。OP农药的尿液代谢物二甲基磷酸酯（DMP）和二甲基硫代磷酸酯（DMTP）在苄基衍生化后通过FPD-GC进行分析。该方法消除了气相色谱图中的干扰峰。发现DMTP的两种异构体衍生物的比例是恒定的。暴露组的DMP和DMTP均显著高于非暴露组，DMP高于DMTP。杀螟硫磷-敌敌畏暴露组的DMP与DMTP之比显著高于杀螟硫磷暴露组。认为该比例可能反映了农药暴露的结果。OP农药的尿液代谢物往往随着上次接触后时间的推移而降低。然而，即使在最后一次接触5天后，仍在少数样本中检测到少量代谢物。
