Zilberstein D, Dwyer D M
Mol Biochem Parasitol. 1984 Jul;12(3):327-36. doi: 10.1016/0166-6851(84)90089-6.
Mid-log phase Leishmania donovani promastigotes accumulated 2-deoxy-D-glucose (2-DOG) via a carrier mediated transport system, maintaining an apparent Km of 24.4 microM and a Vmax of 3.12 nmol mg-1 protein min-1. D-Glucose but not L-glucose competitively inhibited the 2-DOG transport with an apparent Ki of 18.7 microM. Transport of 2-DOG was inhibited by 2,4-dinitrophenol and NaN3. The parasites maintained a 2-DOG gradient of at least 79 fold across the surface membrane, demonstrating the active nature of the transport system.
对数中期的杜氏利什曼原虫前鞭毛体通过载体介导的转运系统积累2-脱氧-D-葡萄糖（2-DOG），其表观米氏常数（Km）为24.4微摩尔，最大反应速度（Vmax）为3.12纳摩尔·毫克⁻¹蛋白质·分钟⁻¹。D-葡萄糖而非L-葡萄糖竞争性抑制2-DOG的转运，其表观抑制常数（Ki）为18.7微摩尔。2,4-二硝基苯酚和叠氮化钠抑制2-DOG的转运。寄生虫在表面膜上维持至少79倍的2-DOG梯度，证明了转运系统的活性本质。
