胆脂瘤和胆固醇肉芽肿的生化研究——δ7-胆甾烯醇在胆脂瘤组织中的出现情况。
Biochemical study of cholesteatoma and cholesterol granuloma--occurrence of delta 7-cholestenol in the tissues of cholesteatoma.
作者信息
Hayashida T, Iwamori M, Kitsuwa T, Nagai Y, Nomura Y
出版信息
ORL J Otorhinolaryngol Relat Spec. 1984;46(5):242-7. doi: 10.1159/000275717.
Qualitative and quantitative analyses of sterol derivatives in the tissue of cholesteatoma and cholesterol granuloma were performed by gas-liquid chromatography, mass spectrometry and mass fragmentography after the extraction of total lipids with organic solvents. Cholesterol was the major component in both tissues. The molar ratios of cholesterol to lipid-bound phosphorus in cholesterol granulomas were higher than those in cholesteatomas. In addition, delta 7-cholestenol (lathosterol) was contained in all tissues of cholesteatoma examined. However, cholesterol granuloma tested did not contain delta 7-cholestenol, indicating that the metabolism of cholesterol differed from that in cholesterol granuloma.
采用气相-液相色谱法、质谱法和质量碎片谱法，在用有机溶剂提取总脂质后，对胆脂瘤和胆固醇肉芽肿组织中的甾醇衍生物进行了定性和定量分析。胆固醇是两种组织中的主要成分。胆固醇肉芽肿中胆固醇与脂质结合磷的摩尔比高于胆脂瘤中的摩尔比。此外，在所检查的所有胆脂瘤组织中均含有Δ7-胆甾烯醇（羊毛甾醇）。然而，所检测的胆固醇肉芽肿中不含Δ7-胆甾烯醇，这表明胆固醇在胆脂瘤中的代谢与在胆固醇肉芽肿中的代谢不同。
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