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两种从不受约束、未受干扰的火鸡身上进行连续采血的方法,并记录急性应激源对催乳素血浆水平的影响。

Two methods for serial blood sampling from unrestrained, undisturbed turkeys with notes on the effects of acute stressors on plasma levels of prolactin.

作者信息

Opel H, Proudman J A

出版信息

Poult Sci. 1984 Aug;63(8):1644-52. doi: 10.3382/ps.0631644.

DOI:10.3382/ps.0631644
PMID:6483727
Abstract

A method was developed for chronic cannulation of the atrium of the turkey. Two methods, one employing a headmount swivel-tethering system and the other a backmount swivel-tethering system, were developed for serial bleeding from cannulated turkeys kept in an environmental cubicle. To validate the methods for serial bleeding from undisturbed, unrestrained turkeys, the effects were examined of several stressors on plasma prolactin (PRL) levels in adult toms. Serial bleeding at 2-min intervals followed by withdrawal of one sample of 50 ml at 2 hr after initiation of bleeding had no effect on plasma PRL. Water deprivation for 24 hr or intraatrial infusion of hypertonic saline had no effect on plasma PRL. Intraatrial injection of 10 mg/kg of epinephrine bitartrate resulted in a significant elevation of plasma concentrations of PRL.

摘要

开发了一种用于火鸡心房慢性插管的方法。开发了两种方法,一种采用头部安装的旋转系留系统,另一种采用背部安装的旋转系留系统,用于对饲养在环境舱中的插管火鸡进行连续采血。为了验证从不受干扰、不受约束的火鸡进行连续采血的方法,研究了几种应激源对成年雄火鸡血浆催乳素(PRL)水平的影响。以2分钟的间隔进行连续采血,在开始采血2小时后抽取一份50毫升的样本,对血浆PRL没有影响。24小时禁水或心房内注入高渗盐水对血浆PRL没有影响。心房内注射10毫克/千克的酒石酸肾上腺素导致血浆PRL浓度显著升高。

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引用本文的文献

1
Plasma levels of arginine vasotocin, prolactin, aldosterone and corticosterone during prolonged dehydration in the domestic fowl: effect of dietary NaCl.
J Comp Physiol B. 1986;156(3):383-97. doi: 10.1007/BF01101101.