Reece W O, Trampel D W, Koehler K J
Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames 50011, USA.
Poult Sci. 2006 Jun;85(6):1095-7. doi: 10.1093/ps/85.6.1095.
The objectives of this research were to study the effect of several time intervals of turkey blood storage from sampling to centrifugation on plasma K, Na, and Cl concentrations and to study the effect of ambient temperature of turkey blood storage on these same variables. In the first study, 6 consecutive blood samples were obtained from each turkey. The first sample was centrifuged immediately (control), and each of the others was respectively stored for 20, 40, 60, 120, or 360 min at 22 degrees C before centrifugation and plasma harvest. In the second study, 4 consecutive blood samples were obtained from each turkey. The first sample was centrifuged immediately (control), and each of the others was respectively stored at ambient temperatures of 9, 22, and 30 degrees C for 120 min before centrifugation and plasma harvest. Plasma K concentration declines and is significant (P < 0.01) for each of the 20, 40, 60, 120, and 360 min values. Plasma Na concentration increases and Cl decreases were not significant (P < 0.01) until 360 and 120 min, respectively. Significant (P < 0.01) reductions occurred for plasma K concentration for each of the ambient temperature values but were the least at 9 degrees C and greatest when stored at 30 degrees C. Plasma Na concentration decreased (P < 0.01) when stored at 9 degrees C. Though increased (P < 0.01) when stored at 22 and 30 degrees C, the difference was not significant (P < 0.01) between samples stored at either 22 or 30 degrees C. Plasma Cl concentration was decreased (P < 0.01) from control values, but there was no significant difference (P < 0.01) among samples stored at 9, 22, and 30 degrees C. Clot formation and its retraction from serum are unduly delayed after blood is withdrawn from turkeys because birds lack the intrinsic mechanism for blood coagulation. Accordingly, serum is not appropriate for the determination of K concentration in turkeys, and plasma harvested immediately after blood sampling is the fluid of choice for K analysis.
本研究的目的是研究火鸡血液从采样到离心的几个时间间隔对血浆钾、钠和氯浓度的影响，以及研究火鸡血液储存环境温度对这些相同变量的影响。在第一项研究中，从每只火鸡身上连续采集6份血样。第一份样本立即离心（对照），其他每份样本在离心和采集血浆前分别在22摄氏度下储存20、40、60、120或360分钟。在第二项研究中，从每只火鸡身上连续采集4份血样。第一份样本立即离心（对照），其他每份样本在离心和采集血浆前分别在9、22和30摄氏度的环境温度下储存120分钟。血浆钾浓度下降，并且在20、40、60、120和360分钟的每个时间点均具有显著性（P<0.01）。血浆钠浓度升高以及氯浓度降低分别在360分钟和120分钟之前均无显著性（P < 0.01）。对于每个环境温度值，血浆钾浓度均出现显著性（P < 0.01）降低，但在9摄氏度时降低最少，在30摄氏度储存时降低最多。当在9摄氏度储存时，血浆钠浓度降低（P < 0.01）。虽然在22和30摄氏度储存时升高（P < 0.01），但在22或30摄氏度储存的样本之间差异不显著（P < 0.01）。血浆氯浓度较对照值降低（P < 0.01），但在9、22和30摄氏度储存的样本之间无显著差异（P < 0.01）。从火鸡采血后，凝血块的形成及其从血清中的回缩会过度延迟，因为鸟类缺乏血液凝固的内在机制。因此，血清不适用于测定火鸡的钾浓度，采血后立即采集的血浆是进行钾分析的首选液体。
