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[14C]尿苷5'-二磷酸和[14C]鸟苷5'-二磷酸的制备

Preparation of [14C]uridine 5'-diphosphate and [14C]guanosine 5'-diphosphate.

作者信息

Cory J G, Bacon P E

出版信息

Prep Biochem. 1984 Aug;14(3):231-7. doi: 10.1080/10826068408070631.

DOI:10.1080/10826068408070631
PMID:6483806
Abstract

Procedures have been developed for the routine enzymatic synthesis of [14C]UDP and [14C]GDP from commercially available enzymes and [14C]UMP and [14C]GMP. Using high pressure liquid chromatography, the products are recovered in high yield (60-80%) and with high purity. The [14C]UDP and [14C]GDP are utilized as substrates for ribonucleotide reductase.

摘要

已经开发出从市售酶以及[14C]UMP和[14C]GMP常规酶促合成[14C]UDP和[14C]GDP的方法。使用高压液相色谱法,产物以高收率(60 - 80%)和高纯度回收。[14C]UDP和[14C]GDP用作核糖核苷酸还原酶的底物。

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