关于大肠杆菌核糖核苷二磷酸还原酶的作用机制。3'-C-H键断裂的证据。
On the mechanism of ribonucleoside diphosphate reductase from Escherichia coli. Evidence for 3'-C--H bond cleavage.
作者信息
Stubbe J, Ackles D
出版信息
J Biol Chem. 1980 Sep 10;255(17):8027-30.
PMID:6997288
Abstract
The 3'-carbon--hydrogen bond of [3'-3H]uridine 5'-diphosphate is cleaved during its conversion to 2'-deoxyuridine 5'-diphosphate catalyzed by Esherichia coli ribonucleoside diphosphate reductase. A selection against 3H of approximately 3.3 is observed on this reduction reaction. During the course of this reaction, a small but significant amount of 3H is released to the solvent.
摘要
在大肠杆菌核糖核苷二磷酸还原酶催化[3'-³H]尿苷5'-二磷酸转化为2'-脱氧尿苷5'-二磷酸的过程中，[3'-³H]尿苷5'-二磷酸的3'-碳氢键被裂解。在该还原反应中观察到约3.3的³H同位素排除。在这个反应过程中，有少量但显著量的³H释放到溶剂中。
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