Dorset D L
Ultramicroscopy. 1984;13(3):311-24. doi: 10.1016/0304-3991(84)90209-2.
Multislice n-beam dynamical calculations have been carried out at 20 and 15.6 A resolution to simulate the electron scattering from a negatively-stained protein microcrystal using a model based on the crystal structure of E. coli matrix porin. Images at t = 100 A, i.e. the thickness of reconstituted vesicle preparation, correspond to the projected potential of the kinematical structure. The kinematical model is valid at least to t = 500 A, beyond which the projected-image symmetry does not correspond to the actual structure. If a crystallographic residual limit R = 0.20 is imposed, the electron diffraction intensity data correspond to the kinematical data up to t = 700 and 1000 A, respectively, for 15.6 and 20 A resolution.
已在20埃和15.6埃分辨率下进行了多切片n束动力学计算，以使用基于大肠杆菌基质孔蛋白晶体结构的模型来模拟从负染色蛋白质微晶的电子散射。在t = 100埃（即重构囊泡制剂的厚度）处的图像对应于运动学结构的投影势。运动学模型至少在t = 500埃时有效，超过该厚度，投影图像对称性与实际结构不对应。如果施加晶体学残余极限R = 0.20，则对于15.6埃和20埃分辨率，电子衍射强度数据分别在t = 700埃和1000埃之前与运动学数据相对应。
