Filion L G, Bielefeldt Ohmann H, Owen P W, Babiuk L A
Vet Immunol Immunopathol. 1984 Aug;7(1):19-32. doi: 10.1016/0165-2427(84)90024-2.
In the present report, the bovine secondary in vitro antibody response to keyhole limpet hemocyanin is described. The induction of anti-KLH antibody was not dependent upon the presence of mitogen but was antigen specific (KLH vs ovalbumin). Furthermore, this response was dependent upon cell density (10(6) per well), antigen dose (1 to 10(-5) ug/culture) and time in culture (5 days). The antibody produced was specific for KLH as measured in several binding assays. An unresponsive state was detected with high concentrations of KLH (more than 10 ug per culture) which was not due to the formation of immune complexes but to the inactivation of B and/or T cells. The induction of the antibody response was dependent on the presence of macrophages (syngeneic or allogeneic) and their presence could not be replaced with 2-mercaptoethanol.
在本报告中，描述了牛对钥孔戚血蓝蛋白的体外二次抗体应答。抗钥孔戚血蓝蛋白抗体的诱导不依赖于有丝分裂原的存在，而是抗原特异性的（钥孔戚血蓝蛋白与卵清蛋白）。此外，这种应答依赖于细胞密度（每孔10⁶个细胞）、抗原剂量（1至10⁻⁵微克/培养物）和培养时间（5天）。在几种结合试验中测定，所产生的抗体对钥孔戚血蓝蛋白具有特异性。在高浓度钥孔戚血蓝蛋白（每培养物超过10微克）时检测到无反应状态，这不是由于免疫复合物的形成，而是由于B和/或T细胞的失活。抗体应答的诱导依赖于巨噬细胞（同基因或异基因）的存在，并且它们的存在不能被2-巯基乙醇替代。
