Kaz'mina Iu G, Salamatova S A, Liubinskaia M M, Chernokhvostova E V
Zh Mikrobiol Epidemiol Immunobiol. 1984 Aug(8):83-8.
The possibility of inducing systemic tolerance in animals by feeding them with ovalbumin and human serum was studied on mice, rats and rabbits. Antibodies to ovalbumin, human serum albumin and immunoglobulins (IgG, IgA, IgM) were determined by the passive hemagglutination test in the sera of the test and control animals after the second immunization made through a parenteral route. Tolerance to all the antigens under study was obtained in mice and rats, while in rabbits such feeding was found to produce the priming effect. The degree of tolerance was the greater, the more was the dose of the antigen and the longer was the period of feeding. Different proteins showed varying tolerogenic activity; the same degree of tolerance in mice was obtained by feeding them with IgG in a dose of 0.3-0.5 mg and with ovalbumin or human serum albumin in a dose of 6-12 mg (per gram of body weight). Tolerance was determined on day 3 after the course of feeding was over; in 3 weeks tolerance essentially decreased, and in 1.5-2 months it was replaced by normal reactiveness. Tolerance induced by the oral administration of antigens proved to be immunologically specific.
通过给小鼠、大鼠和兔子喂食卵清蛋白和人血清来诱导动物产生全身耐受性的可能性进行了研究。在通过非肠道途径进行第二次免疫后，通过被动血凝试验测定试验组和对照组动物血清中针对卵清蛋白、人血清白蛋白和免疫球蛋白（IgG、IgA、IgM）的抗体。在小鼠和大鼠中获得了对所有研究抗原的耐受性，而在兔子中，这种喂食被发现会产生启动效应。耐受性程度越高，抗原剂量越大，喂食期越长。不同蛋白质显示出不同的致耐受性活性；通过给小鼠喂食剂量为0.3 - 0.5毫克的IgG以及剂量为6 - 12毫克（每克体重）的卵清蛋白或人血清白蛋白，可在小鼠中获得相同程度的耐受性。在喂食过程结束后第3天测定耐受性；3周后耐受性基本下降，1.5 - 2个月后被正常反应性所取代。经口服抗原诱导的耐受性被证明具有免疫特异性。
