一种用于特异性测定金黄色葡萄球菌脂肪酶活性的简单比浊法。
A simple turbidimetric method for specific measurement of Staphylococcus aureus lipase activity.
作者信息
Rollof J, Hedström S A, Nilsson-Ehle P
出版信息
Acta Pathol Microbiol Immunol Scand B. 1984 Jun;92(3):155-8. doi: 10.1111/j.1699-0463.1984.tb02811.x.
Abstract
We have developed a simple semiquantitative method for measuring Staphylococcus aureus lipase activity. Enzymatic activity is measured as the decrease in spectrophotometric absorbancy in a lipid emulsion after incubation with the sample. The sensitivity, precision and accuracy are adequate for routine purposes. The assay is rapid and simple and does not require advanced technical equipment.
摘要
我们开发了一种用于测量金黄色葡萄球菌脂肪酶活性的简单半定量方法。酶活性通过与样品孵育后脂质乳剂中分光光度吸光度的降低来测量。该方法的灵敏度、精密度和准确度适用于常规用途。该检测方法快速简便，不需要先进的技术设备。
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