Fassoulaki A, Eger E I, Johnson B H, Ferrell L D, Smuckler E A, Cahalan M K, Eger R R, Harper M H
Anesth Analg. 1984 Oct;63(10):888-90.
We speculated that the inhibitory effect of isoflurane on the metabolism of halothane might reduce hepatic injury produced by halothane. To test this hypothesis we pretreated male rats with phenobarbital and 24 hr later exposed them to one of three types of anesthesia. One group of rats was anesthetized with 0.6 MAC isoflurane in 21-25% oxygen for 20 min, followed by exposure to 0.3 MAC isoflurane and 0.3 MAC halothane either in 9% oxygen for 46 min (n = 5) or in 12% oxygen for 60 min (n = 11). A second group of rats received 0.3 MAC halothane in 9% oxygen for either 46 min (n = 12) or in 12% oxygen for 60 min (n = 10). The third group received 0.3 MAC isoflurane in 9% oxygen for either 46 min (n = 12) or for 120 min (n = 8). The rats were killed 24 hr after the exposures and liver slides prepared. Histologic examination revealed that rats treated with isoflurane plus halothane, or with halothane alone showed a significant hepatic injury (P less than 0.005) when compared with those treated with isoflurane. Thus isoflurane failed to protect the liver from halothane-induced injury.
我们推测，异氟烷对氟烷代谢的抑制作用可能会减轻氟烷所致的肝损伤。为验证这一假设，我们先用苯巴比妥对雄性大鼠进行预处理，24小时后让它们接受三种麻醉方式之一。一组大鼠先在21%-25%氧气中用0.6 MAC异氟烷麻醉20分钟，然后在9%氧气中分别用0.3 MAC异氟烷和0.3 MAC氟烷暴露46分钟（n = 5）或60分钟（n = 11）。第二组大鼠在9%氧气中接受0.3 MAC氟烷，暴露46分钟（n = 12）或60分钟（n = 10）。第三组大鼠在9%氧气中接受0.3 MAC异氟烷，暴露46分钟（n = 12）或120分钟（n = 8）。暴露24小时后处死大鼠并制备肝脏切片。组织学检查显示，与接受异氟烷处理的大鼠相比，接受异氟烷加氟烷处理或仅接受氟烷处理的大鼠出现了明显的肝损伤（P < 0.005）。因此，异氟烷未能保护肝脏免受氟烷诱导的损伤。
