Petrov V V, Ratner E N, Severin A I, Fikhte B A, Kulaev I S
Prikl Biokhim Mikrobiol. 1990 May-Jun;26(3):413-21.
The effect of the bacteriolytic enzyme preparation, lysoamidase, on Staphylococcus aureus 209P cells was studied. The protoplast formation was examined by spectrophotometric, biochemical and electron microscopic methods. Optimal conditions for isolation of S. aureus protoplasts were chosen. The susceptibility of S. aureus cells to lysoamidase depended on the culture age: the maximum effect was observed in the logarithmic growth phase. The protoplast yield was 80% when 1 M sucrose was used as an osmotic stabilizer. Lysoamidase caused local disruptures of the staphylococcus cell walls, which resulted in the formation of osmotically fragile spheroplasts and the release of protoplasts into the medium. The protoplasts obtained could retain 85-90% of the respiration activity and were able of cell wall regeneration.
研究了溶菌酶制剂溶菌酰胺酶对金黄色葡萄球菌209P细胞的作用。通过分光光度法、生化法和电子显微镜法检测原生质体的形成。选择了分离金黄色葡萄球菌原生质体的最佳条件。金黄色葡萄球菌细胞对溶菌酰胺酶的敏感性取决于培养时间：在对数生长期观察到最大效果。当使用1M蔗糖作为渗透稳定剂时，原生质体产量为80%。溶菌酰胺酶导致葡萄球菌细胞壁局部破坏，从而形成渗透脆弱的球状体，并使原生质体释放到培养基中。获得的原生质体可保留85-90%的呼吸活性，并能够进行细胞壁再生。
