Ohnishi S T, Flick J L, Rubin E
Arch Biochem Biophys. 1984 Sep;233(2):588-94. doi: 10.1016/0003-9861(84)90483-1.
The effects of ethanol on both Ca2+ pump activity and Ca2+-induced Ca2+ release in sarcoplasmic reticulum (SR) of rabbit skeletal muscle were studied. In concentrations of 0.1-1.0%, ethanol conspicuously enhanced Ca2+ release from the heavy fraction of SR, whereas a much smaller effect was noted in the light fraction. When Ca2+-induced Ca2+ release was inhibited by 10 mM Mg2+, the Ca2+ pump activities of both the heavy and light SR were the same; the activities were not significantly influenced by 1% ethanol. Ethanol itself did not release Ca2+ from the heavy SR. However, it appeared to render the heavy SR more permeable to Ca2+, thereby decreasing the amount of storable Ca2+. This action of ethanol may be related to the mechanism of its negative inotropic effect.
研究了乙醇对兔骨骼肌肌浆网（SR）中Ca2+泵活性和Ca2+诱导的Ca2+释放的影响。在浓度为0.1 - 1.0%时，乙醇显著增强了SR重组分中的Ca2+释放，而在轻组分中观察到的影响要小得多。当Ca2+诱导的Ca2+释放被10 mM Mg2+抑制时，重、轻SR的Ca2+泵活性相同；1%乙醇对活性没有显著影响。乙醇本身不会从重SR中释放Ca2+。然而，它似乎使重SR对Ca2+的通透性更高，从而减少了可储存Ca2+的量。乙醇的这种作用可能与其负性肌力作用机制有关。
