Bohnensack R
Biomed Biochim Acta. 1984;43(4):403-11.
Rate equations describing the dependence of mitochondrial respiration or ATP formation on the extramitochondrial ATP/ADP ratio were derived from a simplified reaction scheme of oxidative phosphorylation. The equations include maximum velocities of (1) respiration, (2) ATP formation, and (3) other energy utilizing side reactions; apparent Michaelis constants for ATP and ADP; an apparent cooperativity coefficient; and in addition an apparent equilibrium constant which is equal to the ATP/ADP ratio at zero net formation of ATP in the resting state of mitochondria. The control characteristics described by the rate equation are in close agreement with curves that can be obtained from a much more complex mathematical model of phosphorylating mitochondria. The kinetic parameters of the equation can be estimated from experimental data by a nonlinear regression procedure. The results demonstrate a weak apparent cooperativity and a strong shift of the apparent affinity ratio for ATP and ADP towards ADP.
描述线粒体呼吸或ATP生成对外线粒体ATP/ADP比率依赖性的速率方程，是从氧化磷酸化的简化反应方案推导出来的。这些方程包括：（1）呼吸、（2）ATP生成以及（3）其他能量利用副反应的最大速度；ATP和ADP的表观米氏常数；一个表观协同系数；此外还有一个表观平衡常数，它等于线粒体静息状态下ATP净生成量为零时的ATP/ADP比率。速率方程所描述的控制特性与从一个更为复杂的磷酸化线粒体数学模型中得到的曲线密切吻合。该方程的动力学参数可通过非线性回归程序从实验数据中估算出来。结果表明存在微弱的表观协同性，且ATP和ADP的表观亲和力比率明显向ADP偏移。
