Pattinson N R, Chapman B A
Biochem Int. 1984 Aug;9(2):137-42.
To define the efficiency of hepatic detoxification, 14C lithocholate in combination with taurocholate was continuously infused intravenously into rats until steady-state. Quantitation of hepatic radiolabelled bile acid under these conditions showed only 11% of total liver bile acid was unmetabolised, indicating very efficient detoxification of lithocholate, in its most hepatotoxic state. Interestingly we found the rate for each bile acid to reach steady-state differed. To test whether the difference was due to glutathione S-transferase binding, as proposed by Strange et al (8), glutathione S-transferase levels were induced by phenobarbitone treatment. An increase in cytosol glutathione S-transferase levels had no effect on the time it took for each bile acid to reach steady-state.
为了确定肝脏解毒的效率，将14C石胆酸盐与牛磺胆酸盐联合持续静脉输注到大鼠体内，直至达到稳态。在这些条件下对肝脏放射性标记胆汁酸的定量分析表明，肝脏总胆汁酸中只有11%未被代谢，这表明在其最具肝毒性的状态下，石胆酸盐的解毒效率非常高。有趣的是，我们发现每种胆汁酸达到稳态的速率不同。为了测试这种差异是否如斯特兰奇等人（8）所提出的那样是由于谷胱甘肽S-转移酶结合所致，通过苯巴比妥处理诱导谷胱甘肽S-转移酶水平。胞质谷胱甘肽S-转移酶水平的升高对每种胆汁酸达到稳态所需的时间没有影响。
