Ward J A, Roberts R J
Biochim Biophys Acta. 1984 Oct 24;796(1):42-50. doi: 10.1016/0005-2760(84)90236-4.
The effects of breathing greater than 95% oxygen from birth for 48 h of life on surfactant phosphatidylcholine synthesis and secretion, as well as uptake and stability of exogenous phosphatidylcholine were studied using rabbit lung tissue slices. Lung slices from animals breathing greater than 95% oxygen for 48 h exhibited a decreased rate of [14C]phosphatidylcholine release (30%) in comparison to lung slices from air-exposed controls. In vitro incorporation of [14C]choline into phosphatidylcholine was decreased by a similar amount in lung slices from pups exposed to greater than 95% oxygen. Uptake of exogenous [14C]phosphatidylcholine by lung slices from hyperoxic-exposed and control groups was not different, and the stability of extracellular phosphatidylcholine was likewise unaffected by hyperoxia. Turnover of labelled phosphatidylcholine taken up by tissue slices from medium was apparently decreased in association with hyperoxic exposure. These results are consistent with multiple sites of effect of hyperoxia on the pulmonary surfactant system in the newborn. These effects of hyperoxia on the lung surfactant system occur at a time of critical adaption to extrauterine life, and thus may have major consequences on lung function and ultimate survival of the premature infant with respiratory distress syndrome.
利用兔肺组织切片，研究了从出生起呼吸含氧量超过95%的氧气48小时对表面活性物质磷脂酰胆碱合成与分泌以及外源性磷脂酰胆碱摄取与稳定性的影响。与暴露于空气中的对照组动物的肺切片相比，呼吸含氧量超过95%的氧气48小时的动物的肺切片显示[14C]磷脂酰胆碱释放率降低（30%）。在暴露于含氧量超过95%的氧气的幼崽的肺切片中，[14C]胆碱体外掺入磷脂酰胆碱的量也有类似程度的减少。高氧暴露组和对照组的肺切片对外源性[14C]磷脂酰胆碱的摄取没有差异，细胞外磷脂酰胆碱的稳定性同样不受高氧影响。与高氧暴露相关，组织切片从培养基中摄取的标记磷脂酰胆碱的周转率明显降低。这些结果与高氧对新生儿肺表面活性物质系统的多部位作用一致。高氧对肺表面活性物质系统的这些影响发生在对宫外生活的关键适应期，因此可能对患有呼吸窘迫综合征的早产儿的肺功能和最终存活产生重大影响。
