Eidne K A, Bass N M, Sherman M, Millar R P, Kirsch R E
Biochim Biophys Acta. 1984 Oct 16;801(3):424-8. doi: 10.1016/0304-4165(84)90148-x.
Ligandin, a ubiquitous multifunctional cytoplasmic protein which exhibits glutathione S-transferase, glutathione peroxidase and delta 5-3-ketosteroid isomerase activities and binds to cortisol metabolites, is present in relatively high concentrations in gonadal and adrenal tissue. In contrast to hepatic ligandin, little is known about the ontogeny of ligandin in steroid-synthesising tissues. We report here the intracellular concentrations of ligandin as well as the serum concentrations of testosterone and progesterone measured by radioimmunoassay at different stages of development in the rat. Ligandin levels in testis, ovary and adrenal tissue were relatively high soon after birth, decreased by day 9 and increased rapidly during puberty to reach adult levels. These changes appeared to be paralleled by changes in the circulating levels of testosterone and progesterone. In contrast, ligandin levels in non-steroidogenically active tissues, such as liver and kidney, were low at birth and rose progressively to reach adult levels. Whereas hepatic ligandin concentration could be increased at all stages of development by phenobarbital induction, no induction occurred in the endocrine tissues.
配体蛋白是一种普遍存在的多功能细胞质蛋白，具有谷胱甘肽S -转移酶、谷胱甘肽过氧化物酶和δ5 - 3 -酮类固醇异构酶活性，并能与皮质醇代谢产物结合，在性腺和肾上腺组织中的浓度相对较高。与肝脏中的配体蛋白不同，关于配体蛋白在类固醇合成组织中的个体发生知之甚少。我们在此报告了通过放射免疫测定法在大鼠发育的不同阶段测得的配体蛋白的细胞内浓度以及睾酮和孕酮的血清浓度。睾丸、卵巢和肾上腺组织中的配体蛋白水平在出生后不久相对较高，到第9天时下降，并在青春期迅速升高至成年水平。这些变化似乎与睾酮和孕酮的循环水平变化平行。相比之下，非类固醇生成活性组织（如肝脏和肾脏）中的配体蛋白水平在出生时较低，并逐渐升高至成年水平。虽然苯巴比妥诱导可在发育的各个阶段增加肝脏中的配体蛋白浓度，但在内分泌组织中未发生诱导作用。
