Smith D E, Kronenberg M S
Biol Reprod. 1984 Oct;31(3):531-5. doi: 10.1095/biolreprod31.3.531.
Interference of Cu++ with the initial events in estrogen action was tested by determining Cu++ effects on estradiol-receptor interactions. When immature rat uteri were incubated in vitro with [3H] estradiol ([3H]E2), steroid was bound in cytoplasmic fractions and rapidly accumulated in the nuclear fraction in a manner which was dependent upon time and hormone concentration. Uteri which were preincubated with 2 X 10(-4) M CuCl2 for 40-60 min and then exposed to [3H]E2 were found to have a 30-50% decrease in the amount of steroid bound in the cytoplasmic and nuclear fractions. When copper-treated uteri were exposed to [3H]E2 for variable times, the quantity of steroid bound in the cytoplasmic fraction was markedly depressed and the rate of nuclear accumulation of [3H]E2 was significantly decreased. These results show that Cu++ can inhibit [3H]E2 binding to tissue cytoplasmic receptors in vitro and thereby interfere with hormone delivery to target cell nuclei.
通过测定铜离子对雌二醇 - 受体相互作用的影响，来测试铜离子对雌激素作用初始事件的干扰。当未成熟大鼠子宫在体外与[3H]雌二醇（[3H]E2）一起孵育时，类固醇在细胞质部分结合，并以依赖于时间和激素浓度的方式迅速在细胞核部分积累。预先用2×10（-4）M氯化铜孵育40 - 60分钟，然后暴露于[3H]E2的子宫，其细胞质和细胞核部分结合的类固醇量减少了30 - 50％。当用铜处理的子宫在不同时间暴露于[3H]E2时，细胞质部分结合的类固醇量明显降低，并且[3H]E2的核积累速率显著下降。这些结果表明，铜离子在体外可抑制[3H]E2与组织细胞质受体的结合，从而干扰激素向靶细胞核的传递。
