Marcusson J O, Morgan D G, Winblad B, Finch C E
Brain Res. 1984 Oct 8;311(1):51-6. doi: 10.1016/0006-8993(84)91397-0.
Serotonin (S-2) binding sites were characterized in human frontal cortex and hippocampus throughout the lifespan. We found that the S-2 binding sites (labeled by [3H]spiperone and displaced by ketanserin) consisted of two subtypes (S-2A and S-2B) which are discriminated by competition with methysergide. The total S-2 sites in frontal cortex had a Bmax of 360 fmol/mg protein, which is approximately twice that of the hippocampus (160 fmol/mg protein). The density of total S-2 sites decreased with age in the frontal cortex of normal adults (17-100 years, n = 24). The receptor loss was primarily in the S-2A subclass. This loss of S-2A sites occurred primarily after 60 years and decreased to 50% of young adult values by the 10th decade. In the hippocampus, S-2A binding sites decreased with age, but no effects on total S-2 or S-2B sites were detected. A study of infant brains suggested the S-2A subtype in frontal cortex increases postnatally. However, in the infant hippocampus the S-2 binding approximated adult levels.
在整个生命周期中，对人类额叶皮质和海马体中的5-羟色胺（S-2）结合位点进行了表征。我们发现，S-2结合位点（由[³H]螺哌隆标记并被酮色林取代）由两种亚型（S-2A和S-2B）组成，这两种亚型可通过与麦角新碱的竞争来区分。额叶皮质中的总S-2位点的Bmax为360 fmol/mg蛋白质，约为海马体（160 fmol/mg蛋白质）的两倍。正常成年人（17 - 100岁，n = 24）额叶皮质中总S-2位点的密度随年龄增长而降低。受体损失主要发生在S-2A亚类中。S-2A位点的这种损失主要发生在60岁以后，到第十个十年时降至年轻成年人水平的50%。在海马体中，S-2A结合位点随年龄增长而减少，但未检测到对总S-2或S-2B位点的影响。一项对婴儿大脑的研究表明，额叶皮质中的S-2A亚型在出生后增加。然而，在婴儿海马体中，S-2结合接近成人水平。
