Métézeau P, Bernheim A, Berger R, Goldberg M E
Cytometry. 1984 Sep;5(5):550-2. doi: 10.1002/cyto.990050520.
A system is described which allows us to collect flow cytometry sorted objects onto a very small area, thus obtaining very concentrated particles. For that purpose, collection is made directly on a nitrocellulose filter while a vacuum is established below the filter. By comparison with collection onto a microscope slide, the local concentration is increased about 1,000-fold. This efficient and simple system allows us to concentrate cell or cellular organelle populations for observation by microscopy of many objects in a single field, or to study the biological or biochemical properties of a small number of sorted particles.
本文描述了一种系统，该系统使我们能够将流式细胞术分选的物体收集到非常小的区域上，从而获得高度浓缩的颗粒。为此，直接在硝酸纤维素滤膜上进行收集，同时在滤膜下方形成真空。与收集到显微镜载玻片上相比，局部浓度提高了约1000倍。这种高效且简单的系统使我们能够浓缩细胞或细胞器群体，以便通过显微镜观察单个视野中的许多物体，或者研究少量分选颗粒的生物学或生化特性。
