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合成的供体和受体剪接位点在RNA聚合酶B（II）转录单元中起作用。

Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit.

作者信息

Rautmann G, Matthes H W, Gait M J, Breathnach R

出版信息

EMBO J. 1984 Sep;3(9):2021-8. doi: 10.1002/j.1460-2075.1984.tb02085.x.

DOI:10.1002/j.1460-2075.1984.tb02085.x
PMID:6489319
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC557637/
Abstract

We have synthesised a 32-bp oligonucleotide containing sequences conforming to the consensus sequences for donor and acceptor splice sites. The oligonucleotide has been inserted into an RNA polymerase B (II) transcription unit and the resulting recombinant used to study the splicing mechanism. Our findings are as follows: (i) the synthetic sites function when separated by several different prokaryotic or eukaryotic DNA fragments providing bulk intron sequence, (ii) intron size need not be greater than 29 bp, (iii) an AG dinucleotide 11 bp upstream from the invariant AG of an acceptor splice site renders the latter non-functional, and (iv) sequence changes distant from splice sites can affect the efficiency of their utilisation.

摘要

我们合成了一段32个碱基对的寡核苷酸，其包含与供体和受体剪接位点的共有序列相符的序列。该寡核苷酸已被插入到RNA聚合酶B（II）转录单元中，并且所得的重组体被用于研究剪接机制。我们的研究结果如下：（i）当被几个不同的原核或真核DNA片段隔开以提供大量内含子序列时，合成位点起作用；（ii）内含子大小不必大于29个碱基对；（iii）受体剪接位点不变的AG上游11个碱基对处的AG二核苷酸使后者失去功能；（iv）远离剪接位点的序列变化会影响它们的利用效率。

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1
Synthetic donor and acceptor splice sites function in an RNA polymerase B (II) transcription unit.合成的供体和受体剪接位点在RNA聚合酶B（II）转录单元中起作用。
EMBO J. 1984 Sep;3(9):2021-8. doi: 10.1002/j.1460-2075.1984.tb02085.x.
2
Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts.酵母RNA聚合酶II转录本剪接所需的内含子包含序列的证据。
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3
Splice-site signals of mRNA precursors as revealed by computer search. Site-specific mutagenesis and thalassemia.通过计算机搜索揭示的mRNA前体的剪接位点信号。位点特异性诱变与地中海贫血。
J Biochem. 1985 Apr;97(4):1173-9. doi: 10.1093/oxfordjournals.jbchem.a135162.
4
The length of the downstream exon and the substitution of specific sequences affect pre-mRNA splicing in vitro.下游外显子的长度以及特定序列的替换会在体外影响前体mRNA的剪接。
Mol Cell Biol. 1988 Feb;8(2):860-6. doi: 10.1128/mcb.8.2.860-866.1988.
5
Splice site selection and ribonucleoprotein complex assembly during in vitro pre-mRNA splicing.体外前体mRNA剪接过程中的剪接位点选择与核糖核蛋白复合体组装
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Splicing in Caenorhabditis elegans does not require an AG at the 3' splice acceptor site.秀丽隐杆线虫中的剪接在3'剪接受体位点不需要AG。
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RNA splice site selection: evidence for a 5' leads to 3' scanning model.RNA剪接位点选择：5'至3'扫描模型的证据
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A role for branchpoints in splicing in vivo.分支点在体内剪接中的作用。
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5' cleavage site in eukaryotic pre-mRNA splicing is determined by the overall 5' splice region, not by the conserved 5' GU.真核生物前体mRNA剪接中的5'切割位点由整个5'剪接区域决定，而非保守的5'GU。
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Exon tethering in transcription by RNA polymerase II.RNA聚合酶II转录过程中的外显子拴系
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Mismatch extension during strong stop strand transfer and minimal homology requirements for replicative template switching during Moloney murine leukemia virus replication.莫洛尼鼠白血病病毒复制过程中强终止链转移期间的错配延伸以及复制模板切换的最小同源性要求。
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Rapid synthesis of oligodeoxyribonucleotides VI. Efficient, mechanised synthesis of heptadecadeoxyribonucleotides by an improved solid phase phosphotriester route.寡脱氧核糖核苷酸的快速合成VI。通过改进的固相磷三酯路线高效、机械化合成十七聚脱氧核糖核苷酸。
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