Barrow K D, Collins J G, Rogers P L, Smith G M
Eur J Biochem. 1984 Nov 15;145(1):173-9. doi: 10.1111/j.1432-1033.1984.tb08537.x.
A novel polysaccharide has been observed in vivo by 13C nuclear magnetic resonance (NMR) spectroscopy of the gram-negative bacterium, Zymomonas mobilis. The polysaccharide, which was not removed from the Z. mobilis cell by washing with 0.86% saline, was extracted by mild acid treatment. The structure was elucidated by a combination of NMR techniques, including proton and proton-carbon two-dimensional methods. The structure was determined to be -alpha-fructofuranosyl-(2-1)-beta-fructofuranosyl-(2-6)-. The polysaccharide is unlike levan, a beta-(2-6)-fructose polymer which is produced by many bacteria, including Z.mobilis, when grown on sucrose. The polysaccharide isolated here is produced by cells cultured on glucose, fructose or sucrose.
通过对革兰氏阴性细菌运动发酵单胞菌进行碳-13核磁共振（NMR）光谱分析，在体内观察到了一种新型多糖。用0.86%的盐水洗涤无法从运动发酵单胞菌细胞中去除该多糖，通过温和的酸处理将其提取出来。结合包括质子和质子-碳二维方法在内的核磁共振技术阐明了其结构。确定该结构为-α-呋喃果糖基-（2-1）-β-呋喃果糖基-（2-6）-。该多糖不同于果聚糖，果聚糖是一种β-（2-6）-果糖聚合物，许多细菌包括运动发酵单胞菌在蔗糖上生长时都会产生这种聚合物。这里分离出的多糖是由在葡萄糖、果糖或蔗糖上培养的细胞产生的。
