Jousimaa J, Merenmies J, Rauvala H
Eur J Cell Biol. 1984 Sep;35(1):55-61.
A method using proteins covalently coupled to glass surfaces has been applied to studies on the differentiation of neuroblasts. Neuroblastoma cells from clones N 18 and NIE 115 adhere to surfaces coated with fibronectin or laminin and extend rapidly growth cone-containing neurites. Some lectin-coated surfaces are also able to support neurite outgrowth, although the activities are lower than those of fibronectin and laminin. We discuss the biochemical requirements of the surface structures capable of inducing a differentiated neuronal morphology to neuroblastoma cells, and also consider the possible relationship of the results to physiological differentiation phenomena.
一种将蛋白质共价偶联到玻璃表面的方法已应用于神经母细胞分化的研究。来自克隆N 18和NIE 115的神经母细胞瘤细胞粘附于涂有纤连蛋白或层粘连蛋白的表面，并迅速伸出含有生长锥的神经突。一些凝集素包被的表面也能够支持神经突生长，尽管其活性低于纤连蛋白和层粘连蛋白。我们讨论了能够诱导神经母细胞瘤细胞分化为神经元形态的表面结构的生化要求，并考虑了这些结果与生理分化现象之间可能的关系。
