Liesi P, Dahl D, Vaheri A
J Neurosci Res. 1984;11(3):241-51. doi: 10.1002/jnr.490110304.
Neurons, mechanically dissociated from newborn rat brain and identified by immunostaining for neurofilaments, attached preferentially to laminin-coated coverslips without need of an underlying glial monolayer. The most extensive neurite outgrowth was seen when 20-30 micrograms/ml of laminin was used to coat the coverslips. Higher concentrations of laminin (greater than 30 micrograms/ml) supported single neurons to spread on the coverslips. Fibronectin coating of the coverslips allowed glial cells to attach more rapidly than on uncoated surfaces, but it did not support neuronal spreading or neurite outgrowth. Spreading of neurons and neurite outgrowth were completely inhibited by preincubation of laminin-coated coverslips with laminin antibodies but were unaffected by fibronectin antibodies. These results indicate that laminin is an attachment and spreading factor for central neurons in culture and suggest the presence of a laminin receptor on the neuronal cell surfaces.
从新生大鼠大脑中机械分离出来并通过神经丝免疫染色鉴定的神经元，优先附着于层粘连蛋白包被的盖玻片上，而无需底层的神经胶质单层。当使用20 - 30微克/毫升的层粘连蛋白包被盖玻片时，观察到最广泛的神经突生长。更高浓度的层粘连蛋白（大于30微克/毫升）支持单个神经元在盖玻片上伸展。盖玻片用纤连蛋白包被时，神经胶质细胞比在未包被的表面上附着得更快，但它不支持神经元伸展或神经突生长。用层粘连蛋白抗体预孵育层粘连蛋白包被的盖玻片可完全抑制神经元的伸展和神经突生长，但纤连蛋白抗体对此无影响。这些结果表明层粘连蛋白是培养的中枢神经元的附着和伸展因子，并提示神经元细胞表面存在层粘连蛋白受体。
