Wahl R L, Sherman P, Fisher S
Eur J Nucl Med. 1984;9(8):382-4. doi: 10.1007/BF00252876.
Monoclonal antibodies are assuming increasing importance in experimental and clinical medicine. Generally, tissue biodistribution studies in animals precede human studies. To investigate a concern of ours that varying methods of sample handling in these studies could result in apparent alterations in tissue-binding levels, we compared two methods of tissue processing after the administration of labeled antibodies: one including only blotting away of blood, the other involving several washing steps. The unwashed, blotted specimens were found to have significantly more radioactivity per gram of tissue than the washed, ranging from 22% more in the spleen to 52% more in the lungs and left ventricle. Since in vivo imaging is dependent on the total mount of radioactivity in an organ, we believe the most meaningful determination of tissue radioactivity should be based on unwashed samples. Awareness of this problem is suggested to allow meaningful extrapolations from measured tissue localization data to imaging and therapy.
单克隆抗体在实验医学和临床医学中发挥着越来越重要的作用。一般来说，在人体研究之前会先在动物身上进行组织生物分布研究。为了探究我们所担心的这些研究中不同的样本处理方法可能导致组织结合水平出现明显变化的问题，我们比较了给予标记抗体后两种组织处理方法：一种仅包括吸干血液，另一种涉及多个洗涤步骤。结果发现，每克组织中未洗涤、吸干的标本的放射性比洗涤后的标本显著更多，脾脏中多22%，肺和左心室中多52%。由于体内成像取决于器官中的放射性总量，我们认为对组织放射性最有意义的测定应该基于未洗涤的样本。建议认识到这个问题，以便能够从测量的组织定位数据进行有意义的推断，用于成像和治疗。
