通过细胞荧光测定法鉴定携带抗体的精子。
Identification of antibody-laden sperm by cytofluorometry.
作者信息
Haas G G, Cunningham M E
出版信息
Fertil Steril. 1984 Oct;42(4):606-13. doi: 10.1016/s0015-0282(16)48146-x.
A direct assay for measuring sperm-associated immunoglobulin G (IgG) and an indirect assay for measuring IgG plasma antisperm antibodies were devised utilizing fluorescein-labeled antihuman IgG and a fluorescein-activated flow cytometer. The method singularly analyzed Ig attached to the surface of sperm because sperm and nonsperm cells were separately assessed. The direct assay technique results correlated with results from a previously reported direct radiolabeled antiglobulin assay. As few as 100,000 sperm are required in the indirect method; the sensitivity of the indirect technique corresponded with that of a previously reported indirect radiolabeled antiglobulin assay.
利用荧光素标记的抗人IgG和荧光素激活的流式细胞仪，设计了一种用于测量精子相关免疫球蛋白G（IgG）的直接检测方法和一种用于测量IgG血浆抗精子抗体的间接检测方法。该方法单独分析附着在精子表面的Ig，因为精子和非精子细胞是分别评估的。直接检测技术的结果与先前报道的直接放射性标记抗球蛋白检测的结果相关。间接方法中所需的精子数量少至100,000个；间接技术的灵敏度与先前报道的间接放射性标记抗球蛋白检测的灵敏度相当。
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