D'Cruz O J, Haas G G, Reichlin M
Department of Obstetrics and Gynecology, University of Oklahoma Health Sciences Center, Oklahoma City.
Fertil Steril. 1994 Oct;62(4):834-44. doi: 10.1016/s0015-0282(16)57013-7.
To evaluate a flow cytometric method to detect and quantitate serum anti-DNA antibodies using unfixed, swollen and decondensed human sperm nuclei and to examine the relationship between antibodies against sperm surface antigens to the presence of antibodies against nuclear antigens.
Serum IgG and IgG subclass antibodies to decondensed sperm nuclei were detected by indirect immunofluorescence (IIF) flow cytometry. Sera were screened by IIF for anti-double-stranded DNA antibodies using the protozoan Crithidia luciliae as the substrate and for antinuclear antibodies using human epithelial (HEp 2) cells, respectively. All sera were assessed for antibodies against the sperm plasma membrane by an indirect immunobead test.
Infertility laboratory at the University of Oklahoma Health Sciences Center and rheumatology laboratory at the Oklahoma Medical Research Foundation.
Sera from 33 antisperm antibody-positive patients (5 subgroups), 33 patients with systemic lupus erythematosus (SLE; 6 subgroups), and 20 normal controls were selected.
IgG antibodies against decondensed sperm nuclear DNA were detected in 11 (33.3%) of 33 antisperm antibody-positive patients versus 14 (42.4%) of 33 patients with SLE. Anti-DNA antibodies were most prevalent in vasectomized men and in antisperm antibody positive women with SLE. In the sera from patients with SLE, the presence of the anti-nuclear ribonucleoprotein antibody was associated with the presence of sperm head-directed antisperm antibodies. Anti-double-stranded DNA antibodies were found in 6 (18.1%) of 33 sera from patients with antisperm antibody and 17 (51.5%) of 33 sera from patients with SLE. Antinuclear antibodies were found in only 9 (27.2%) of 33 sera from patients with antisperm antibody and 30 (90.9%) of 33 sera from patients with SLE. All 20 of the control sera gave negative results in the three tests. Serum IgG reactivity to sperm nuclei was predominantly of the IgG1 and IgG3 subclasses.
Anti-DNA is frequently found in either patients with antisperm antibodies or patients with SLE. Our results indicated that decondensed sperm nuclei can provide a specific substrate for screening serum anti-DNA antibodies.
评估一种流式细胞术方法，该方法使用未固定、肿胀且解聚的人类精子细胞核来检测和定量血清抗DNA抗体，并研究抗精子表面抗原抗体与抗核抗原抗体存在之间的关系。
通过间接免疫荧光（IIF）流式细胞术检测血清IgG和IgG亚类抗体与解聚精子细胞核的反应。分别使用原生动物利什曼原虫作为底物，通过IIF筛查血清中的抗双链DNA抗体，使用人上皮（HEp-2）细胞筛查抗核抗体。通过间接免疫珠试验评估所有血清中针对精子质膜的抗体。
俄克拉荷马大学健康科学中心的不孕症实验室和俄克拉荷马医学研究基金会的风湿病实验室。
选取了33例抗精子抗体阳性患者（5个亚组）、33例系统性红斑狼疮（SLE；6个亚组）患者和20例正常对照的血清。
33例抗精子抗体阳性患者中有11例（33.3%）检测到针对解聚精子核DNA的IgG抗体，而33例SLE患者中有14例（42.4%）检测到。抗DNA抗体在输精管切除的男性和患有SLE的抗精子抗体阳性女性中最为常见。在SLE患者的血清中，抗核糖核蛋白抗体的存在与精子头部定向抗精子抗体的存在相关。33例抗精子抗体患者的血清中有6例（18.1%）检测到抗双链DNA抗体，33例SLE患者的血清中有17例（51.5%）检测到。33例抗精子抗体患者的血清中只有9例（27.2%）检测到抗核抗体，33例SLE患者的血清中有30例（90.9%）检测到。所有20例对照血清在三项检测中均呈阴性结果。血清IgG与精子细胞核的反应主要为IgG1和IgG3亚类。
抗精子抗体患者或SLE患者中经常发现抗DNA抗体。我们的结果表明，解聚的精子细胞核可为筛查血清抗DNA抗体提供一种特异性底物。
