Pittman D, Ernst S G
Dev Biol. 1984 Nov;106(1):236-42. doi: 10.1016/0012-1606(84)90078-2.
Strongylocentrotus purpuratus embryos were fractionated into two cell populations of defined lineages at times corresponding to two critical developmental events: determination (16-cell stage) and early differentiation (mesenchyme blastula). The 16-cell stage blastomeres, labeled with [35S]methionine, exhibited identical protein synthesis patterns by fluorography, and this pattern was not significantly altered by cell separation. In comparing the proteins of the mesenchyme blastula to the 16-cell stage, differences (increases and decreases) were seen by fluorography of newly synthesized proteins. The synthesis of 2.9% of the mesenchyme blastula proteins is specific to or enriched in primary mesenchyme cells and 8.2% is specific to or enriched in endoderm/ectoderm cells. Additionally, in contrast to the earlier stage, the pattern of protein synthesis in the mesenchyme blastula embryos is substantially altered by cell separation. The ability to alter protein synthesis in response to environmental factors may be a further demonstration of the differentiation of these cells.
决定期（16细胞期）和早期分化期（间充质囊胚）。用[35S]甲硫氨酸标记的16细胞期卵裂球，通过荧光自显影显示出相同的蛋白质合成模式，并且这种模式不会因细胞分离而发生显著改变。在将间充质囊胚的蛋白质与16细胞期的蛋白质进行比较时，通过对新合成蛋白质的荧光自显影观察到了差异（增加和减少）。间充质囊胚中2.9%的蛋白质合成是初级间充质细胞特有的或在其中富集的，8.2%是内胚层/外胚层细胞特有的或在其中富集的。此外，与早期阶段相反，间充质囊胚胚胎中的蛋白质合成模式会因细胞分离而发生显著改变。响应环境因素改变蛋白质合成的能力可能是这些细胞分化的进一步证明。
