• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一个编码与脊椎动物中H2A.F/Z组蛋白相关的海胆组蛋白H2A变体的cDNA克隆的特征分析。

Characterization of a cDNA clone coding for a sea urchin histone H2A variant related to the H2A.F/Z histone protein in vertebrates.

作者信息

Ernst S G, Miller H, Brenner C A, Nocente-McGrath C, Francis S, McIsaac R

出版信息

Nucleic Acids Res. 1987 Jun 11;15(11):4629-44. doi: 10.1093/nar/15.11.4629.

DOI:10.1093/nar/15.11.4629
PMID:2438657
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC340885/
Abstract

A cDNA clone coding for a sea urchin histone H2A variant has been isolated. The coding region of the clone has been sequenced and the sequence found to be closely related to the H2A.F sequence in chickens. The nucleotide sequence of the sea urchin H2A.F/Z is 74% conserved when compared to chicken H2A.F and 51% conserved compared to sea urchin H2A early and 60% compared to sea urchin H2A late. The nucleotide-derived amino acid comparisons show that H2A.F/Z is 97% homologous with H2A.F in chickens and 57% and 56% homologous when compared to sea urchin H2A early and late respectively. There are between 3-6 copies of the H2A.F/Z sequence in the S. purpuratus genome. The H2A.F/Z gene sequence codes for the previously identified H2A.Z protein. All embryonic stages and adult tissues tested contain mRNA for H2A.F/Z. The mRNA appears in the poly A+ RNA fraction after chromatography over oligo dT cellulose.

摘要

一个编码海胆组蛋白H2A变体的cDNA克隆已被分离出来。该克隆的编码区已被测序，发现其序列与鸡的H2A.F序列密切相关。与鸡H2A.F相比，海胆H2A.F/Z的核苷酸序列保守性为74%；与海胆早期H2A相比为51%，与海胆晚期H2A相比为60%。从核苷酸推导的氨基酸比较表明，H2A.F/Z与鸡的H2A.F同源性为97%，与海胆早期和晚期H2A相比分别为57%和56%同源。在紫球海胆基因组中，H2A.F/Z序列有3至6个拷贝。H2A.F/Z基因序列编码先前鉴定的H2A.Z蛋白。所有测试的胚胎阶段和成年组织都含有H2A.F/Z的mRNA。该mRNA在经寡聚dT纤维素层析后出现在多聚A+RNA组分中。

相似文献

1
Characterization of a cDNA clone coding for a sea urchin histone H2A variant related to the H2A.F/Z histone protein in vertebrates.一个编码与脊椎动物中H2A.F/Z组蛋白相关的海胆组蛋白H2A变体的cDNA克隆的特征分析。
Nucleic Acids Res. 1987 Jun 11;15(11):4629-44. doi: 10.1093/nar/15.11.4629.
2
Synthesis of sperm and late histone cDNAs of the sea urchin with a primer complementary to the conserved 3' terminal palindrome: evidence for tissue-specific and more general histone gene variants.用与保守的3'末端回文序列互补的引物合成海胆精子和晚期组蛋白cDNA：组织特异性和更普遍的组蛋白基因变体的证据。
Proc Natl Acad Sci U S A. 1985 Sep;82(17):5676-80. doi: 10.1073/pnas.82.17.5676.
3
Histone H2A.F/Z mRNA is stored in the egg cytoplasm and basally regulated in the sea urchin embryo.组蛋白H2A.F/Z信使核糖核酸存储于卵子细胞质中，并在海胆胚胎中受到基础调控。
Dev Biol. 1992 Oct;153(2):402-6. doi: 10.1016/0012-1606(92)90125-z.
4
Polyadenylylation of sea urchin histone RNA sequences in transfected COS cells.转染的COS细胞中海胆组蛋白RNA序列的聚腺苷酸化
Proc Natl Acad Sci U S A. 1985 Feb;82(4):1094-8. doi: 10.1073/pnas.82.4.1094.
5
Drosophila has a single copy of the gene encoding a highly conserved histone H2A variant of the H2A.F/Z type.果蝇有一个编码H2A.F/Z型高度保守组蛋白H2A变体的基因的单拷贝。
Nucleic Acids Res. 1988 Aug 11;16(15):7487-97. doi: 10.1093/nar/16.15.7487.
6
The DNA sequence of sea urchin (S. purpuratus) H2A, H2B and H3 histone coding and spacer regions.海胆（紫球海胆）H2A、H2B和H3组蛋白编码区及间隔区的DNA序列。
Cell. 1978 Nov;15(3):1033-44. doi: 10.1016/0092-8674(78)90287-8.
7
Characterization of two nonallelic pairs of late histone H2A and H2B genes of the sea urchin: differential regulation in the embryo and tissue-specific expression in the adult.海胆晚期组蛋白H2A和H2B基因的两个非等位基因对的特征：胚胎中的差异调控和成体中的组织特异性表达。
Mol Cell Biol. 1986 Nov;6(11):3746-54. doi: 10.1128/mcb.6.11.3746-3754.1986.
8
Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species.哺乳动物H2A.Z的cDNA序列，H2A.Z是一种在进化上有差异但高度保守的基础组蛋白H2A同工型。
Nucleic Acids Res. 1988 Feb 11;16(3):1113-24. doi: 10.1093/nar/16.3.1113.
9
The nucleotide and amino acid coding sequence of a gene for H1 histone that interacts with euchromatin. The early embryonic H1 gene of the sea urchin Strongylocentrotus purpuratus.与常染色质相互作用的H1组蛋白基因的核苷酸和氨基酸编码序列。紫海胆早期胚胎H1基因。
J Biol Chem. 1982 Aug 25;257(16):9438-43.
10
Isolation of a new H3.3 histone variant cDNA of P. lividus sea urchin: sequence and embryonic expression.紫球海胆新H3.3组蛋白变体cDNA的分离：序列与胚胎表达
Biochim Biophys Acta. 1994 Oct 18;1219(2):539-42. doi: 10.1016/0167-4781(94)90084-1.

引用本文的文献

1
Widespread priming of transcriptional regulatory elements by incipient accessibility or RNA polymerase II pause in early embryos of the sea urchin Strongylocentrotus purpuratus.海胆 Strongylocentrotus purpuratus 早期胚胎中初生可及性或 RNA 聚合酶 II 暂停对转录调控元件的广泛启动作用。
Genetics. 2023 Oct 4;225(2). doi: 10.1093/genetics/iyad145.
2
Quantitative developmental transcriptomes of the sea urchin Strongylocentrotus purpuratus.海胆 Strongylocentrotus purpuratus 的定量发育转录组。
Dev Biol. 2014 Jan 15;385(2):160-7. doi: 10.1016/j.ydbio.2013.11.019. Epub 2013 Nov 26.
3
H2A.Z has a function reminiscent of an activator required for preferential binding to intergenic DNA.

本文引用的文献

1
Histone variants specific to the transcriptionally active, amitotically dividing macronucleus of the unicellular eucaryote, Tetrahymena thermophila.组蛋白变体特异性存在于单细胞真核生物嗜热栖热四膜虫转录活跃、无丝分裂的大核中。
Cell. 1980 Jul;20(3):609-17. doi: 10.1016/0092-8674(80)90307-4.
2
Simultaneous synthesis, translation, and storage of mRNA including histone mRNA in sea urchin eggs.海胆卵中包括组蛋白mRNA在内的mRNA的同步合成、翻译和储存。
Dev Biol. 1980 Sep;79(1):139-48. doi: 10.1016/0012-1606(80)90079-2.
3
Differential conservation of histone 2A variants between mammals and sea urchins.
H2A.Z具有一种功能，类似于优先结合基因间DNA所需的激活因子。
EMBO J. 2003 Sep 1;22(17):4512-22. doi: 10.1093/emboj/cdg427.
4
The nonessential H2A N-terminal tail can function as an essential charge patch on the H2A.Z variant N-terminal tail.非必需的H2A N端尾部可作为H2A.Z变体N端尾部上的必需电荷补丁发挥作用。
Mol Cell Biol. 2003 Apr;23(8):2778-89. doi: 10.1128/MCB.23.8.2778-2789.2003.
5
Growth regulation of human variant histone genes and acetylation of the encoded proteins.人类变异组蛋白基因的生长调控及编码蛋白的乙酰化作用
Mol Biol Rep. 2000 Jun;27(2):61-71. doi: 10.1023/a:1007156629024.
6
The five cleavage-stage (CS) histones of the sea urchin are encoded by a maternally expressed family of replacement histone genes: functional equivalence of the CS H1 and frog H1M (B4) proteins.海胆的五种卵裂期（CS）组蛋白由一个母源表达的替代组蛋白基因家族编码：CS H1和青蛙H1M（B4）蛋白的功能等效性。
Mol Cell Biol. 1997 Mar;17(3):1189-200. doi: 10.1128/MCB.17.3.1189.
7
H2A.ZI, a new variant histone expressed during Xenopus early development exhibits several distinct features from the core histone H2A.H2A.ZI是非洲爪蟾早期发育过程中表达的一种新型变体组蛋白，它具有一些与核心组蛋白H2A不同的显著特征。
Nucleic Acids Res. 1996 Oct 15;24(20):3947-52. doi: 10.1093/nar/24.20.3947.
8
Essential and nonessential histone H2A variants in Tetrahymena thermophila.嗜热四膜虫中的必需和非必需组蛋白H2A变体
Mol Cell Biol. 1996 Aug;16(8):4305-11. doi: 10.1128/MCB.16.8.4305.
9
Either of the major H2A genes but not an evolutionarily conserved H2A.F/Z variant of Tetrahymena thermophila can function as the sole H2A gene in the yeast Saccharomyces cerevisiae.嗜热四膜虫的两个主要H2A基因中的任何一个，但不是进化保守的H2A.F/Z变体，都可以在酿酒酵母中作为唯一的H2A基因发挥作用。
Mol Cell Biol. 1996 Jun;16(6):2878-87. doi: 10.1128/MCB.16.6.2878.
10
Histone H2A.X gene transcription is regulated differently than transcription of other replication-linked histone genes.组蛋白H2A.X基因的转录调控方式与其他与复制相关的组蛋白基因的转录不同。
Mol Cell Biol. 1993 Feb;13(2):984-92. doi: 10.1128/mcb.13.2.984-992.1993.
哺乳动物和海胆之间组蛋白2A变体的差异保守性。
J Cell Biol. 1982 May;93(2):426-31. doi: 10.1083/jcb.93.2.426.
4
Nucleosome structure.核小体结构。
Annu Rev Biochem. 1980;49:1115-56. doi: 10.1146/annurev.bi.49.070180.005343.
5
Amino acid sequence of the N-terminal domain of calf thymus histone H2A.Z.小牛胸腺组蛋白H2A.Z N端结构域的氨基酸序列
FEBS Lett. 1983 Apr 5;154(1):166-70. doi: 10.1016/0014-5793(83)80896-5.
6
The organization and expression of histone gene families.组蛋白基因家族的组织与表达
Cell. 1981 Aug;25(2):301-13. doi: 10.1016/0092-8674(81)90048-9.
7
H2A.F: an extremely variant histone H2A sequence expressed in the chicken embryo.H2A.F：一种在鸡胚胎中表达的极其变异的组蛋白H2A序列。
Proc Natl Acad Sci U S A. 1983 May;80(10):2819-23. doi: 10.1073/pnas.80.10.2819.
8
Developmental time, cell lineage, and environment regulate the newly synthesized proteins in sea urchin embryos.发育时间、细胞谱系和环境调节海胆胚胎中新合成的蛋白质。
Dev Biol. 1984 Nov;106(1):236-42. doi: 10.1016/0012-1606(84)90078-2.
9
Sea urchin (lytechinus pictus) late-stage histone H3 and H4 genes: characterization and mapping of a clustered but nontandemly linked multigene family.海胆（艳丽刺海胆）晚期组蛋白H3和H4基因：一个成簇但非串联连接的多基因家族的特征与定位
Cell. 1982 Dec;31(2 Pt 1):383-93. doi: 10.1016/0092-8674(82)90132-5.
10
Distinct organizations and patterns of expression of early and late histone gene sets in the sea urchin.海胆早期和晚期组蛋白基因集的不同组织及表达模式。
Nature. 1983 Jan 13;301(5896):120-5. doi: 10.1038/301120a0.