Deane D L, Cohen B B, Morton J E, Steel C M
Int J Cancer. 1984 Oct 15;34(4):459-62. doi: 10.1002/ijc.2910340405.
Five human lymphoblastoid cell lines which have become tumorigenic, as judged by transplantability into immunosuppressed mice, have been compared with earlier, non-tumorigenic stocks of the same cultures, recovered from liquid nitrogen. Analysis of the cell surface glycoproteins by SDS-PAGE, electro-transfer to cellulose nitrate and "blotting" with radioiodinated lectins, reveals an increase in the molecular weight of one band from around 190 kd to 220 kd, in each case coinciding with the acquisition of the tumorigenic phenotype. Probing the electrotransfers with a monoclonal antibody demonstrates that the altered glycoprotein is a member of the "leukocyte common antigen" family. The increase in molecular weight is due to the addition of carbohydrate residues which are removed by treatment with neuraminidase and endoglycosidase. Screening a larger number of lymphoid cell lines, including those derived from Burkitt's lymphoma, shows an excellent correlation between tumorigenicity and altered molecular weight of a leukocyte common antigen.
通过移植到免疫抑制小鼠体内判断，有五种人淋巴母细胞系已具有致瘤性，将它们与从液氮中复苏的同一培养物早期的非致瘤性细胞株进行了比较。通过SDS - PAGE分析细胞表面糖蛋白，电转移至硝酸纤维素膜并用放射性碘化凝集素“印迹”，结果显示在每种情况下，有一条带的分子量从约190kd增加到220kd，这与致瘤表型的获得恰好同时发生。用单克隆抗体探测电转移膜表明，改变的糖蛋白是“白细胞共同抗原”家族的一员。分子量的增加是由于添加了碳水化合物残基，这些残基可被神经氨酸酶和内切糖苷酶处理去除。对大量淋巴样细胞系进行筛选，包括源自伯基特淋巴瘤的细胞系，结果显示致瘤性与白细胞共同抗原分子量的改变之间存在极好的相关性。
